緑色光タンパク質の光化学に関する構造的洞察:ヒスタグのユニークな役割

Alan Deng1, Steven G Boxer1

  • 1Department of Chemistry, Stanford University , Stanford, California 94305-5012, United States.

まとめ

オリゴヒスティジンタグ (Hisタグ) は,代替ベータ鎖として作用することで,切断された緑色光タンパク質 (GFP) を予期せぬ形で安定させる. この構造的役割は様々な性質を説明し,光遺傳学的応用を可能にします.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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