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関連する概念動画

Protein Folding01:22

Protein Folding

Overview
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
Protein Folding01:22

Protein Folding

Overview
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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環状パルミュートされたサブユニットを用いたタンパク質ケージ構造の多様化

Yusuke Azuma1, Michael Herger1, Donald Hilvert1

  • 1Laboratory of Organic Chemistry, ETH Zurich , 8093 Zurich, Switzerland.

Journal of the American Chemical Society
|December 20, 2017
PubMed
まとめ

研究者らは,循環的に変異したルマジン合成酵素 (cpAaLS) タンパク質を設計し,多用途のタンパク質ケージを作りました. この新しい構成要素は 調節可能な構造と バイオテクノロジーや医学における 多様な応用を可能にします

科学分野:

  • バイオテクノロジー
  • タンパク質工学
  • ナノテクノロジー

背景:

  • 自己組み立てたタンパク質ケージは バイオテクノロジーと医学にとって 価値のあるナノスケール容器です
  • 自然のケージタンパク質をカスタマイズし 新しいタンパク質を設計することは その有用性を拡大するために不可欠です

研究 の 目的:

  • プロテインのナノコンパートメントを 作るための 汎用的なビルディングブロックを設計する
  • ケージタンパク質の特性を調整する円形変異の可能性を調査する.

主な方法:

  • Aquifex aeolicus lumazine synthase (AaLS) の円形変異によりcpAaLSが生成される.
  • cpAaLSを球形と管状のケージに自己組み立て
  • cpAaLSと野生型の AaLSの共同生産により,パッチワークのケージを形成する.

主要な成果:

  • cpAaLSは制御可能な球形や管状のケージ構造に自己組み立てられます.
  • コアセンブリによって形成されたパッチワークのケージは,ゲストタンパク質の封じ込めと外部の修正を可能にします.
  • この戦略は,コンパートメントのサイズと静電性を調整することを可能にします.

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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結論:

  • 円形パルムテッド AaLS (cpAaLS) は,タンパク質ナノコンパートメントのための多用途な構成要素を提供します.
  • コアセンブリは 機能的で 適したタンパク質ケージを 作り出す方法を提供します
  • 円形変異は タンパク質ケージ工学の有望な戦略です