![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
光によるタンパク質構成変化の誘導:ヘテロビメタル酸化酵素における光誘導による構造再編成
Pearson T Maugeri1, Julia J Griese2, Rui M Branca3
1Biophysics Graduate Program, The Ohio State University , Columbus, Ohio 43210, United States.
Journal of the American Chemical Society
|December 23, 2017
まとめ
R2loxタンパク質は 光を利用して 化学反応を起こし 鉄の中心を変化させ 二酸化炭素を放出します この発見は 生物学と環境の浄化における 新しい応用の扉を開くのです
科学分野:
- 生物化学
- 写真化学
- 酵素学
背景:
- R2loxは酸素を活性化し,C−H結合の酸化を行う異金属酵素である.
- 活性部位の近くで独特のチロシン-バリンクロスリンクを形成する.
研究 の 目的:
- R2loxタンパク質の活性部位における光誘発変化を調査する.
- 光で活性化されたR2lox種の構造とメカニズムを特徴付ける.
主な方法:
- スペクトロスコーピテクニック (例えば,UV-Vis,EPR)
- X線結晶学
- マススペクトロメトリー
- サイト指向型変異
主要な成果:
- R2loxは光に誘発された変化を経験し,チロシネートに結合した鉄の中心を形成します.
- これはグルタミン酸残基のデカルボキシル化とチロシン- ヴァリンクロスリンクの割れ目を伴う.
- このプロセスは,バイオレットと近紫外線で高量子効率を示し,リガンドから金属への電荷移転によって開始されます.
結論:
- 光変換されたR2lox種は,光誘発デカルボキシル化とクロスリンク割れによるものです.
- タイロシン162は,クロスリンクに関与する調整残留物として特定されています.
- R2loxの光誘発デカルボキシル化能力は,制御された化学合成と環境修復における潜在的な応用を示唆する.
関連する概念動画
01:58Cooperative Allosteric Transitions
9.0K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
9.0K
01:58Cooperative Allosteric Transitions
2.7K
2.7K
01:58Cooperative Allosteric Transitions
3.1K
3.1K
01:26Photochemical Electrocyclic Reactions: Stereochemistry
2.3K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
Selection Rules: Photochemical Activation
2.3K
01:13Introduction to Mechanisms of Enzyme Catalysis
10.9K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
10.9K
02:34Structural Isomerism
21.8K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.8K