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RNA Interference01:23

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Dicerは,dsRNA末端を認識するために異なるモジュールを使用します.

Niladri K Sinha, Janet Iwasa, Peter S Shen

  • 1Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA. bbass@biochem.utah.edu peter.shen@biochem.utah.edu.

Science (New York, N.Y.)
|December 23, 2017
PubMed
まとめ
この要約は機械生成です。

ドロソフィラDicer-2は,そのヘリケースドメインを使用して,これまで知られていなかった抗ウイルス防御のメカニズムである鈍い二重鎖RNA (dsRNA) 末端を結合します. この解き放つとスレッドするプロセスは,効果的な免疫のためにdsRNAの分裂を最適化します.

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科学分野:

  • 分子生物学
  • ウイルス学
  • 構造生物学

背景:

  • 脊椎動物は,Dicer酵素を用いてウイルスの二重鎖RNA (dsRNA) を処理する.
  • ドロソフィラDicer-2は,dSRNA末端に基づく基板差別を示し,分裂パターン (漸進対分布) に影響する.
  • 以前は,Platform-PAZドメインがDicerにおける唯一のdsRNA端末結合部位であると考えられていた.

研究 の 目的:

  • ドロソフィラDicer-2のdSRNA端末間の差別化の構造的基礎を解明する.
  • dsRNA基質結合と分裂における異なるDicerドメインの役割を調査する.
  • 抗ウイルス防御の新たなメカニズムを 発見するためです

主な方法:

  • 高解像度構造を決定するために,冷凍電子顕微鏡 (cryo-EM) が使用されました.
  • ドロソフィラDicer-2の構造は,単体および複合体で,ぼんやりしたdsRNAで解明されました.
  • アデノシントリフォスファート (ATP) 依存解体を評価するために生化学的測定法を使用した.

主要な成果:

  • 以前はターミナル結合に関与しなかったヘリケーゼドメインは,ぼんやりしたdsRNAの結合に不可欠である.
  • ぼんやりとしたdsRNAは局所的に解き放たれ,ATPに依存した方法でヘリケースドメインを通過します.
  • 3' 上の dsRNA 末端はヘリコースドメインを結合させない.

結論:

  • dsRNA terminiの認識とDicer-2による処理に関与する新しいメカニズムが発見されました.
  • この発見は 抗ウイルス性先天性免疫の 新しい調節層を明らかにしています
  • この研究は,他のDicerのオルトログおよび関連する酵素におけるヘリケーゼ依存機能の探求への道を開く.