孔環境制御と共性有機フレームワークに浸透した酵素の性能向上
Qi Sun1, Chung-Wei Fu1,2, Briana Aguila1
1Department of Chemistry, University of South Florida , 4202 E. Fowler Avenue, Tampa, Florida 33620, United States.
Journal of the American Chemical Society
|December 26, 2017
まとめ
同性有機フレームワーク (COF) は,持続可能な化学製造のための酵素の安定性と活性性を高めます. これらのCOF酵素複合物は,従来の生物触媒と比較して優れた性能と再利用性を提供しています.
科学分野:
- 材料科学
- 生物化学
- 化学工学
背景:
- 生物触媒は化学薬品の製造に持続可能な経路を提供しますが,しばしば動作の安定性,限られた動作範囲,低回転性があります.
- 酵素の不動化は,これらの制限を克服し,実用的なアプリケーションを可能にするために不可欠です.
研究 の 目的:
- 酵素の不動化のための理想的な宿主物質として共性有機フレームワーク (COF) を調査する.
- COFを用いて酵素,特にリパースPSの安定性,強度,および活性を増強する.
主な方法:
- COFマトリックス内の酵素固定.
- COF酵素バイオ複合物の製造
- アルコールアッセイの運動解像度による酵素活性と安定性の評価
- メソポール性シリカと金属有機構造で固定された酵素との比較.
主要な成果:
- COFリパスのPSバイオ複合物は,自由酵素と比較して,著しく高い安定性と強度を示した.
- 固定された酵素は,メソポラスシリカと金属有機構造の酵素を上回る優れた活性を示した.
- 調節可能なCOFの毛穴環境は,酵素活性部位の指向を調節し,酵素活性をさらに改善しました.
- よく定義されたCOFチャネルは,反応剤のアクセシビリティを改善し,酵素を無効化から保護し,厳しい環境での耐性を高めました.
結論:
- 結合有機フレームワーク (COF) は,酵素不動化のための非常に有望な宿主材料です.
- COFベースの生物触媒は,持続的な化学合成のために,安定性,活性,および再利用性を向上させます.
- COFの設計ルールをさらに理解することで,簡単に入手可能な"棚"のバイオカタリストの開発につながる可能性があります.
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