活体細胞におけるATPセンシングのためのアップコンバーション発光活性化DNAナノデバイス

Jian Zhao¹, Jinhong Gao¹, Wenting Xue¹

¹CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , Beijing 100190, China.

Journal of the American Chemical Society

|December 28, 2017

まとめ

生体細胞のATPを検出するための DNAナノデバイスを開発しましたこの装置は近赤外線 (NIR) を使って,センサーを一時的に制御し,細胞内の標的検出を可能にします.

科学分野:

バイオテクノロジー
ナノテクノロジー
分子生物学

背景:

DNAナノデバイスは細胞の標的を検出する可能性を秘めています
生命体内のDNAセンサを遠隔操作で活性化することはまだ課題です

研究の目的:

細胞内でATPを検出する DNAナノデバイスを開発する
リモート刺激を用いて感知活動に時間的な制御を達成する.

主な方法:

合成DNAナノデバイスを設計し紫外線で活性化できるアプタマープローブを組み込みました
ナノトランスデューサとしてランタン化ドーピングアップコンバージョンナノ粒子を利用した.
装置の起動と制御のために近赤外線 (NIR) を使います.

主要な成果:

アプタマー探査機の効率的な細胞配送を証明した
NIR光照射によるATPの光センサーの時間制御を達成した.
ナノデバイスの機能を in vitroとin vivoで検証した.

結論:

開発されたDNAナノデバイスは,生きている細胞でNIRトリガーされたATP検出を可能にします.
このプラットフォームはセンサーの時間制御と生物学的機能の潜在的調節を可能にします
多用途のプラットフォームは,様々なアプタマーを使用して,多様な標的を感知するために適応することができます.

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