哺乳類の宿主における微生物の非侵襲的なイメージングのための音響レポーター遺伝子
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Raymond W Bourdeau1, Audrey Lee-Gosselin1, Anupama Lakshmanan2
1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Nature
|January 5, 2018
まとめ
研究者たちは 超音波を用いて 身体の奥深くにある 改造された細菌を視覚化するために 音響レポーター遺伝子を開発しました この画期的な発見は 現在のイメージング方法の限界を克服し 微生物群のよりよい研究と 微生物療法の開発を可能にします
科学分野:
- 微生物学と生物医学工学
- 合成生物学と医学イメージング
背景:
- 哺乳類の微生物群は 健康や病気に 重要な役割を果たします
- 微生物の位置を in vivo で画像化するための現在の方法 (光学レポーター,放射性トレーサ) は,深層組織への浸透と解像度において制限があります.
- 微生物の正確な局所化は 微生物療法と診断の開発に不可欠です
研究 の 目的:
- 遺伝子組み換え微生物細胞の非侵襲的な深層組織イメージングのための新しい方法を導入する.
- 哺乳類の微生物群と人工微生物を研究するための既存のイメージング技術の限界を克服する.
主な方法:
- 超音波で視覚化できる タンパク質ナノ構造を基にした 音響レポーター遺伝子の開発
- エシェリキア・コライやサルモネラ・タイフィムリウムのような細菌におけるガスの膀遺伝子群の異質表現.
- 消化器系と腫瘍の局所化のための概念証明モデルにおける in vivo 画像の実証.
主要な成果:
- 高解像度 (<100μm) の超音波を用いて,ガスを発現する合成細菌を非侵襲的に視覚化しました.
- 画像は低体積密度 (0. 01%以下) で得られた.
- 異なる細菌集団の複合画像を可能にする,音響的に異なるレポーターが開発された.
結論:
- 音響レポーター遺伝子は 哺乳類の宿主体内にある 微生物の細胞を視覚化するための 強力な新しいツールを提供します
- この技術は,哺乳類の微生物群の研究と先進的な微生物診断と治療法の開発を容易にする.
- 超音波による画像処理は 低コストで高解像度な 細胞画像処理の代替手段です
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