タンパク質の機能的な風景を探求するためのエムルションにおける多重複合遺伝子合成

Calin Plesa¹, Angus M Sidore², Nathan B Lubock¹

¹Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.

Science (New York, N.Y.)

|January 6, 2018

まとめ

私たちはDropSynthを開発しました何千もの合成遺伝子を安く作る新しい方法ですこのツールは遺伝子機能の大規模テストを可能にし,生物学的発見を加速します.

科学分野:

合成生物学
分子生物学
ゲノミクス

背景:

DNA配列の効率的な構築と機能的特徴化は,生物学的研究にとって極めて重要です.
大規模な遺伝子合成と機能テストの現在の方法は,費用がかかり,時間がかかります.

研究の目的:

ドロップシンスを導入する. ゲノム長さのDNA構造の複合合成のためのスケーラブルで低コストな方法.
遺伝子ホモログの機能を分析することで,大規模な機能ゲノミクスのためのDropSynthの有用性を実証する.

主な方法:

ドロップシンスはバーコード付きビーズを使って遺伝子組み立てに必要なオリゴヌクレオチドを捕捉します
オリゴヌクレオチドは加工され,油中の水溶液の中で遺伝子構造に組み込まれます.
*エシェリキア・コライ*における合成遺伝子の補完活性をテストするために,多重機能アッセイが行われました.

主要な成果:

ドロップシンスを使って 7000以上の遺伝子構造を合成しました
エシェリキア・コーリ (Escherichia coli) の重要な遺伝子の遺伝学的に多様な同類種を特定した.
マルチプレックス・テストによる遺伝子同類の機能的不適合性の主要な機能的動機と理由を決定する.

結論:

DropSynthは,合成遺伝子構築のためのスケーラブルで費用対効果の高いプラットフォームを提供します.
ドロップシンスをマルチプレックス機能アッセイと組み合わせることでシーケンス-機能関係に関する前例のない探索が可能になります
このアプローチは遺伝子の機能と進化の理解を加速します

さらに関連する動画

13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.8K

11:26

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Published on: March 21, 2016

15.3K

関連する概念動画

02:11

Proteins: From Genes to Degradation

14.6K

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...

14.6K

02:11

Proteins: From Genes to Degradation

4.6K

01:56

Structural Protein Function

30.1K

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...

30.1K

00:51

Synthesis and Functions of Calcitonin

4.8K

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...

4.8K

01:58

Mechanical Protein Functions

5.7K

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.

5.7K

01:42

What is Gene Expression?

197.2K

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...