イーストのプリオンタンパク質の相分離は細胞の適性を促進する
Titus M Franzmann1, Marcus Jahnel1,2, Andrei Pozniakovsky1
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Science (New York, N.Y.)
|January 6, 2018
まとめ
Sup35のような酵母プリオンタンパク質は ストレス下では保護ゲルを形成し 変換因子の損傷を防ぎます タンパク質の振る舞いを制御することで 細胞の適応を助ける環境センサーとして プリオンドメインを明らかにしています
科学分野:
- 細胞生物学
- 生物化学
- 神経科学
背景:
- プリオンドメインは神経変性疾患において重要な役割を果たしますが,その生理学的役割は不明です.
- Sup35のような酵母プリオンは 自己増殖集積を形成することが知られている.
研究 の 目的:
- イーストのプリオンタンパク質Sup35の生理学的機能を調査する.
- ストレスに対する細胞の反応における プリオンドメインの役割を理解する
主な方法:
- ストレス下でのSup35の行動を研究した.
- 段階分離におけるN端プリオン領域と隣接するpHセンサー領域の役割を調査した.
- Sup35相分離が酵母細胞生存に与える影響を分析した.
主要な成果:
- Sup35は,液体のような相分離と,その後のストレス下での凝結によって,保護性のあるpH調節ゲルを形成します.
- N端のプリオン領域は相分離を介し,隣接するpHセンサー領域はこれを調節する.
- この相分離は,必須のSup35変換因子をストレスによる損傷から保護し,酵母細胞の生存を促進します.
結論:
- プリオンのようなドメインは 保存された環境ストレスセンサーとして機能します
- これらのドメインは,タンパク質の相行動を調節することによって,不安定な環境への迅速な適応を促進します.
- ストレス下でのSup35の相分離は酵母細胞の重要な生存メカニズムです.
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