内生性酸化窒素のインビボ画像化のための比度音響性プローブ
Christopher J Reinhardt1, Effie Y Zhou1, Michael D Jorgensen1
1Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
Journal of the American Chemical Society
|January 10, 2018
まとめ
研究者らは,光音学画像を用いた窒素酸化物 (NO) の感度検知のためのAcoustogenic Probes (APNO) を開発した. 最良の探査機であるAPNO-5は,生体内でのNOの比率測定を可能にし,生物学的研究に希望を示しています.
科学分野:
- バイオメディカルイメージング
- 分子イメージング
- 化学生物学
背景:
- フォトアコースティック (PA) 画像は高解像度で深部組織を可視化します.
- 活性化可能な探査機は,特定の生物学的刺激を in vivo で検出するために不可欠です.
- 窒素酸化物 (NO) は,様々な生理学的および病理学的プロセスにおいて重要な役割を果たします.
研究 の 目的:
- 窒素酸化物 (APNO) の新型音響性探査機を設計し,評価する.
- 選択的なNO検出のための特定の光物理特性を持つ探査機を開発する.
- 活体動物による炎症モデルでのAPNO-5の有用性を実証する.
主な方法:
- 一連のAPNO探査機の合成と特徴付け
- NO反応によるスペクトルシフトを含む光物理学的性質の評価
- ネズミのリポポリサカリド誘発炎症モデルと比率的PA画像を用いたin vivo検証
主要な成果:
- APNO-5はNOに迅速な反応を示し,91nmの低色差を持つN- ニトロゾ製品を形成した.
- APNO-5は,NOに対して適切な光物理的特性,生物適合性,安定性,選択性を示した.
- in vivo試験では,内因的なNO検出のPA信号が1. 9倍,および比率的なオンが1. 3倍であることが示されました.
結論:
- APNO-5は,光音学画像を用いた in vivo NO検出のための非常に効果的な比測定装置です.
- この探査機は,生物学的システムにおけるNOの敏感で選択的な視覚化を可能にします.
- APNO-5は,NO関連の生物医学研究と診断を進めるための大きな可能性を秘めています.
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