Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Phase-lead and Phase-lag Controllers01:22

Phase-lead and Phase-lag Controllers

585
Understanding the working function of different types of controllers can be illustrated with practical analogies, such as adjusting a stereo's volume equalizer. Cranking up the bass involves a phase-lead controller, which functions as a high-pass filter, while increasing the treble uses a phase-lag controller, which acts as a low-pass filter. PD controllers, similar to high-pass filters, enhance the system's response to high-frequency components. PI controllers, akin to low-pass...
585
Peptide Bonds02:43

Peptide Bonds

83.7K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
83.7K
Hybrid Zones02:29

Hybrid Zones

22.0K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
22.0K
Time and frequency -Domain Interpretation of Phase-lead Control01:24

Time and frequency -Domain Interpretation of Phase-lead Control

481
Phase-lead controllers are commonly used in various control systems to enhance response speed and stability. Adjusting the brightness on a television screen offers a practical example of phase-lead control. When contrast is enhanced, a phase-lead controller is employed. Mathematically, phase-lead control is identified when the first parameter is smaller than the second.
The design of phase-lead control involves the strategic placement of poles and zeros to balance steady-state error and system...
481
Time and frequency -Domain Interpretation of Phase-lag Control01:21

Time and frequency -Domain Interpretation of Phase-lag Control

424
Phase-lag controllers are widely used in control systems to improve stability and reduce steady-state errors. A dimmer switch controlling the brightness of a light bulb serves as a practical example of phase-lag control, gradually adjusting the bulb's brightness. Mathematically, phase-lag control or low-pass filtering is represented when the factor 'a' is less than 1.
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any...
424
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

68.0K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
68.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Composite biomaterials of polyelectrolyte complex micelle nanoparticles in hyaluronic acid gels enable local, targeted miR-92a inhibition and enhanced angiogenesis in diabetic wound repair.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Effect of Polymer Structure on the Thermodynamics of Polyelectrolyte Complex Micelle Formation.

Macromolecules·2026
Same author

Influence of Charge Block Length on Conformation and Cluster Formation of Atactic Peptide Polyampholytes.

Macromolecules·2026
Same author

Messenger RNA delivery to islet β cells using conjugated lipid nanoparticles.

Cell reports. Medicine·2026
Same author

Precision mRNA Nanomedicine for Targeted Vascular Therapies in ARDS and Atherosclerosis.

bioRxiv : the preprint server for biology·2026
Same author

Simulation of weak polyelectrolyte brushes: the effects of ionizable monomer fraction and monovalent salt.

Soft matter·2026
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
関連記事をすべて見る

関連する実験動画

Updated: Feb 15, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.5K

オリゴヌクレオチド・ペプチド複合体:ハイブリッド化による相制御

Jeffrey R Vieregg1, Michael Lueckheide2, Amanda B Marciel1

  • 1Institute for Molecular Engineering, University of Chicago , Chicago, Illinois 60637, United States.

Journal of the American Chemical Society
|January 10, 2018
PubMed
まとめ
この要約は機械生成です。

核酸の混合状態は,カチオン性ペプチドと複合すると固体沈殿物または液体コアセルバートを形成するかどうかを決定する. この発見は,治療や感知用ナノ粒子を開発する可能性を秘めています.

さらに関連する動画

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.7K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

関連する実験動画

Last Updated: Feb 15, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.5K
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.7K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

科学分野:

  • ポリマー科学
  • 生物物理化学
  • 分子生物学

背景:

  • 反対に電荷を帯びたポリマー混合物における相分離を駆動する.これは解決されていない重要な問題である.
  • 核酸は,高電荷のポリアニオンで,細胞内の基本的なタンパク質 (クロマチン) を介して凝縮し,膜のない臓器を形成します.
  • 治療用ヌクレア酸を納入するためのナノ粒子の組み立てには 静電相互作用が鍵となります

研究 の 目的:

  • オリゴヌクレオチドとカチオン性ペプチドの間に形成される複合体の相行動を調べる.
  • 核酸の構造と電荷密度が複合体の形成と相分離にどのように影響するかを理解する.
  • これらの複合体の潜在能力を 治療やセンシングの応用に活用する.

主な方法:

  • オリゴヌクレオチド (RNA,メチルフォスフォナート骨幹) とカチオン性ペプチドによる複合化実験
  • ポリマーの長さ,濃度,構造が異なる
  • 塩の濃度が複合相に与える影響を研究する.

主要な成果:

  • 核酸の混合状態は複合体の相を制御する.二重鎖は固体沈殿物を形成し,単一鎖は液体コアセルバートを形成する.
  • 単一鎖のオリゴヌクレオチドの低電荷密度は,液体コアセルバートの形成に寄与する.
  • 塩の添加により,沈殿物はコアセルバットに変化し,コアセルバットに結合したオリゴヌクレオチドはハイブリッド化能力を維持する.

結論:

  • 核酸のハイブリッド化状態は,複雑な相行動の決定要因である.
  • オリゴヌクレオチド複合体は,塩分濃度などの環境刺激に反応して,固体と液体の相の間を切り替えることができる.
  • これらの発見は,高度な応用のための環境に反応する複合体とナノ粒子を創造する可能性を示唆しています.