牛の血清アルブミン・ゴールド複合体の形状変化による光
1Department of Physics and Optical Science, Center for Biomedical Engineering & Science, The University of North Carolina , Charlotte, North Carolina 28223, United States.
Journal of the American Chemical Society
|January 24, 2018
まとめ
牛の血清アルブミン-ゴールド (BSA-Au) 複合体における赤色光は,老化したBSA形状の特定のカチオン黄金結合部位から生じ,Au25ナノクラスターから生じません. この発見は,BSA-Auの複合光メカニズムに新しい視点を提示しています.
科学分野:
- 生物化学
- 材料科学
- ナノテクノロジー
背景:
- 牛の血清アルブミン (BSA) - 金 (Au) 化合物は,以前はAu25ナノクラスターとして記述されていました.
- これらの化合物はナノ粒子に還元可能であり,BSA-cationic Au複合体であることを示唆しています.
研究 の 目的:
- BSAコンフォーメーションとBSA-Au複合体の光性の関係を調査する.
- BSA内の潜在的なカチオンのAU結合部位を特定する.
- BSA-Au複合体の光機構の代替解釈を提案する.
主な方法:
- 試験されたpH誘発および非自然化されたBSA形状.
- BSA-Au複合体の光性能を測定した.
- エキサイテーション・エミッション・マップの測定を用いた.
- 赤色光に対する最低Au (III) の比率を決定した.
主要な成果:
- 赤色光 (λem = 640 nm) は,老化したBSA形状 (pH > 10) でのみ観察された.
- 他のBSA形状 (膨張型,急速型,正常型,基本型) は赤色光を生成しなかった.
- 内部エネルギー伝達メカニズムは赤色光に寄与する可能性があります.
- 赤色光のために必要なAU (III) の最小集合比は7未満でした.
結論:
- BSA-Au複合体の赤色光は,老化したBSAにおける特定のカチオンのAu結合部位と関連している.
- これはニュートラルなAu25ナノクラスターモデルの単一サイト核化に矛盾する.
- BSA内に複数の特定のAu結合部位があり,複雑な光に影響を与えます.
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