単細胞RNA-seqによる心血管系分離の初期段階の定義

Fabienne Lescroart¹, Xiaonan Wang^2,3, Xionghui Lin¹

¹Université Libre de Bruxelles, Laboratory of Stem Cells and Cancer, Brussels B-1070, Belgium.

Science (New York, N.Y.)

|January 27, 2018

まとめ

Mesp1はマウスの心血管前原体 (CP) の発達に不可欠であり,多能細胞から特殊な心臓系統への移行を調節する. この研究では,Mesp1

科学分野:

発達生物学
心血管研究
遺伝学

背景:

マウスの心臓の発達は,ガストルレーション中に特定された,Mesp1を発現する心臓血管前駆体 (CPs) から生じる.
CPの早期の地域および系統分離を制御する分子メカニズムは,まだ完全に理解されていません.

研究の目的:

心血管病原体の早期の地域および系統分離を制御する分子プロセスを解明する.
心臓細胞の特異化と分化におけるMesp1の役割を調査する.

主な方法:

単細胞RNA配列解析 (scRNA-seq) は,野生型およびMesp1-nullのCPをマウスで実施した.
トランスクリプトーム解析は,Mesp1依存型と独立型CP群の遺伝子発現プロフィールを比較するために使用された.

主要な成果:

Mesp1 CPsは分子異質性を示し,エピブラストからメソダーマルおよび血液形成の祖先への連続体上に存在します.
Mesp1は多能状態から抜け出し,心血管の遺伝子発現プログラムを開始するために不可欠です.
特定の心臓の系統と領域に結合した原始体に対応する異なるMesp1 CP集団が特定されました.

結論:

Mesp1は多能性の早期離脱と心臓発育経路の誘導に重要な役割を果たします.
この研究では,マウスのガストルレーション中に早期の系統制限と心臓の祖先の地域分離に関連した分子特性を特定しました.
この発見は,早期の心臓の地域化と血統配分の分子基盤の洞察を提供します.

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