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Published on: April 26, 2018

体細胞核移転によるマカク類のクローン化

Zhen Liu¹, Yijun Cai¹, Yan Wang¹

¹Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, State Key Laboratory of Neuroscience, CAS Key Laboratory of Primate Neurobiology, Chinese Academy of Sciences, Shanghai, China.

|February 4, 2018

まとめ

科学者たちは体細胞核移転 (SCNT) を用いてシノモルガス猿 (Macaca fascicularis) をクローンした. 初期の胚発達の改善は成功率を向上させ,霊長類のモデルを作るためのSCNTの実現可能性を示した.

科学分野:

生殖生物学
発達生物学
遺伝学

背景:

遺伝的に均一な非人間霊長類は生物医学研究や霊長類の生物学を理解するのに価値があります
ソマティック細胞核移転 (SCNT) は,遺伝的に同一の個体を作るための技術です.

研究の目的:

SCNTを用いたシノモルグス猿 (マカカ・ファシキュラリス) のクローニング方法を確立する.
非ヒトの霊長類のSCNTの効率を向上させるため

主な方法:

ソマティック細胞核移転 (SCNT) は,シノモルガス猿の胎児線維芽細胞と成人の結晶細胞を用いて行われました.
改善には,H3K9me3デメチラーゼKdm4dmRNAを注入し,ヒストン脱酸化酵素阻害剤トリコスタチンAを単細胞段階で投与した.
SCNT胚は妊娠のために代理母に移植された.

主要な成果:

胎児の線維芽細胞を用いたSCNTは,6つの妊娠から健康なクローン猿2匹を生成した.
成人クミュルス細胞を用いたSCNTは22回の妊娠から2匹の短命のクローン猿を生成した.
遺伝分析により,核とミトコンドリアDNAは分別,ドナー細胞と卵細胞から発生したことが確認されました.

結論:

SCNTによるマカクのクローン化も可能です特に胎児の線維細胞を用いてです
特定の分子治療による初期の胚発達の最適化は,霊長類におけるSCNTの成功率を大幅に高めます.
この研究は,研究のための遺伝的に均一な霊長類のモデルを生み出すための基礎を提供します.

キーワード:

遺伝子編集ヒトの病気モデルマカクのクローン化人間以外の霊長類体細胞の核移転累積細胞エピジェネティック・モジュール胎児の線維芽細胞霊長類の神経生物学再プログラム

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