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関連する概念動画

Hybrid Zones02:29

Hybrid Zones

22.0K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
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Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

111.6K
Overview
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Ligand Binding Sites02:40

Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Ligand Binding Sites02:40

Ligand Binding Sites

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Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Zones of Protection01:16

Zones of Protection

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In power systems, the entire setup is divided into protective zones to isolate faults and protect the rest of the network. These zones include generators, transformers, buses, transmission lines, distribution lines, and motors. Each zone can be visualized as a separate room in a house, with each room protected by its own circuit breaker.
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...
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Updated: Feb 15, 2026

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
09:37

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Published on: August 31, 2009

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ドーパミンの分泌は,稀な活性ゾーンのような放出部位によって媒介される.

Changliang Liu1, Lauren Kershberg1, Jiexin Wang1

  • 1Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

Cell
|February 6, 2018
PubMed
まとめ

ドーパミンの放出は ストライアタムの特殊な活性ゾーンのような部位を使用し,広範囲に拡散しません. この発見は ドーパミンの正確なシグナル伝達と調節のための 分子機構を明らかにしています

科学分野:

  • 神経科学
  • 分子生物学

背景:

  • ドーパミンは 脳の機能に不可欠で ボリューム伝達で機能します
  • シナプス伝達とは異なり,ドーパミンの放出メカニズムは不明で,組織的な放出部位が欠けていた可能性がある.

研究 の 目的:

  • 線状ドーパミンの分泌が 特殊な放出装置を利用しているかどうかを調べる
  • ドーパミンの放出に関与する分子成分を特定する

主な方法:

  • ドーパミン静脈のタンパク質のコクラスタリングを視覚化するための超高解像度顕微鏡
  • RIMとELKSタンパク質の条件付きノックアウト
  • オプトジェネティック刺激で ドーパミンの放出力学を評価する

主要な成果:

  • ドーパミン静脈症の30%で発見された活性ゾーンタンパク質のコクラスタリング (ファソン,RIM,ELKS).
  • 条件付きRIMノックアウトはドーパミンの放出をなくし,ELKSノックアウトは効果がなかった.
  • オプトジェネティクスは ドーパミンの迅速な放出を 高い確率で確認した

結論:

  • 帯ドーパミンの分泌は,稀少で特化した活性ゾーンのような部位で発生する.
キーワード:
オオカミRIM についてアクティブ・ゾーンバグーンドーパミンエクソサイトーシスストライタムスーパー解像度静脈炎 静脈炎ボリューム伝送

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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

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関連する実験動画

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Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
09:37

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

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  • このメカニズムは ドーパミンの正確な空間時間的コーディングをサポートします
  • 特定のタンパク質の構造は ドーパミンの放出と調節に不可欠です