分子およびナノスケール材料の直接核配送のための光制御ナノプラットフォームの開発
Ya-Xuan Zhu1, Hao-Ran Jia1, Guang-Yu Pan1
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , 2 Sipailou Road , Nanjing 210096 , P. R. China.
Journal of the American Chemical Society
|February 7, 2018
まとめ
研究者らは,多面性オリゴメリックシルセスキオキサン (POSS),ポリエチレングリコール (PEG),ローズベンガル (RB) を使用した新しいナノプラットフォームを開発し,薬物およびナノ材料の光制御核配送を行いました.
科学分野:
- 生物医学工学
- ナノテクノロジー
- 細胞生物学
背景:
- 核膜は,核孔 (9-12 nm) のサイズが限られているため,核を標的とする剤にとって重要な障壁である.
- 化学療法やナノ医療など様々な用途において 効果的な核伝達が不可欠です
研究 の 目的:
- 化学化合物やナノ物質を細胞核に届けるための一般的で制御可能なプラットフォームを開発する.
- 核膜の障壁を克服するために 光誘導メカニズムを使用します
主な方法:
- ポリアミンを含むPOSS,PEG,およびローズベンガル (RB) をナノ粒子 (PPRNP) に自己組み立て
- 光誘発のシングレット酸素生成を利用してリゾソームを破壊し,核膜の浸透を促進する.
- 化学療法剤とナノマテリアルを PPR NPsに納入する.
主要な成果:
- PPR NPsは細胞に内蔵され,リソソームに局所化された.
- 軽度の光照射により,シングレット酸素の生成が引き起こされ,リソソームが破壊され,PPRNPが核膜に蓄積される.
- 化学療法剤 (10-ヒドロキシカンプトセチン,ドセタキセル),光染料 (DiD),ナノ材料 (プルーシアンブルーNP,ゴールドナノ棒) の核投与が成功しました.
- ナノプラットフォームは一般的な適用性を示し,複雑なリガンド改変を回避しました.
結論:
- 開発されたPPRNPプラットフォームは,核の配送に高度に制御可能で一般的なアプローチを提供します.
- この光で活性化された戦略は,分子ペイロードとナノ材料の両方の核膜のバリアを効果的に回避します.
- ナノプラットフォームは 治療と診断の可能性を高める 伝統的な核伝達方法の 約束された代替手段です
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