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強化剤の冗長性により,哺乳類の発達に現象的強度がもたらされる.
Marco Osterwalder1, Iros Barozzi1, Virginie Tissières2,3
1Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
Nature
|February 9, 2018
まとめ
哺乳類のゲノムは多重な機能を提供する多数の遺伝子増強剤を持っています. この遺伝的冗長性は,表型の強さを確保し,個々の強化剤が失われると発達の異常を防止します.
科学分野:
- ゲノミクス
- 発達生物学
- 分子生物学
背景:
- 哺乳類のゲノムには,遺伝子発現を調節する多数の組織特異な増強剤が含まれています.
- この複雑な規制ネットワークの機能的意義,特に強化器の冗長性はよく理解されていません.
研究 の 目的:
- 同じ遺伝子の近くで同様の活動を持つ複数の強化剤の機能的重要性を調査する.
- 強化剤の冗長性が機能喪失変異に表型的強度を与えるかどうかを判断する.
主な方法:
- ゲノム編集を活用して 23のマウス 削除ラインを作りました
- シングルとコンビネタリーなエンハンサーの削除を実行した.
- 29匹のマウスの発達組織から 統合されたエピジェノミクスとトランスクリプトミクスデータ
主要な成果:
- 個々の肢体強化剤の削除は,顕著な形態的変化を引き起こさなかった.
- 肢体増強剤のペアの除去は,機能的な冗長性を示す,識別可能な肢体フェノタイプをもたらした.
- 遺伝子は通常,類似の空間時間的な活動を示す複数の増強剤と関連付けられ,規制的なバッファを提供します.
結論:
- 強化剤の冗長性は哺乳類のゲノムに広く見られる特徴です.
- この冗長性は,個々の強化剤の損失に対するバッファリングによって,現象的強度を提供します.
- 増強剤の冗長性は正常な遺伝子発現を保証し,発達の過程で有害な現象的影響を防ぐ.
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