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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

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This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
18.9K
Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
21.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

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RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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関連する実験動画

Updated: Jan 15, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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人間 で は ない 霊長類 を 複製 する ため の オーダーメイド 卵細胞

Jose B Cibelli1, John B Gurdon2

  • 1Departments of Animal Science and Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.

Cell
|February 10, 2018
PubMed
まとめ

研究者たちは胎児の繊維細胞を使って 2匹のマカクをクローンにしました この突破は,体細胞核移転 (SCNT) のエピジェネティック改変の改善によって達成されました.

科学分野:

  • 生殖生物学
  • 発達生物学
  • 霊長類の研究

背景:

  • 体細胞核移転 (SCNT) は哺乳類のクローン技術である.
  • 霊長類のSCNT効率は,初期の発達障害のために低いままです.
  • SCNTの成功には エピジェネティック再プログラムが不可欠です

研究 の 目的:

  • 類人猿に対してより効率的な SCNT 方法を確立する.
  • SCNTの発達の初期に表遺伝的障壁を克服するために
  • 健康なクローン霊長類を 生み出すために

主な方法:

  • 胎児の線維細胞をドナー細胞として利用した.
  • 人工的に増強されたエピジェネティック・モディファイヤー
  • マカクの体細胞核移転を行った.

主要な成果:

  • 2匹の健康なマカク類のクローンを作りました
  • 強化されたSCNT技術の有効性を証明した.
  • SCNTの早期発達障害を克服しました.

結論:

さらに関連する動画

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関連する実験動画

Last Updated: Jan 15, 2026

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

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  • エピジェネティック・モディフィケーションの強化により,霊長類におけるSCNTの成功率は著しく改善されます.
  • この研究は霊長類のクローン化に 有効な方法を示しています
  • この発見は 霊長類の生殖技術におけるさらなる進歩の道を開きます