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関連する概念動画

Kinetic Energy00:23

Kinetic Energy

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Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
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Enzyme Kinetics01:19

Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
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Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy03:07

Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy

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The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws.
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Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

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Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
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Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)01:16

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

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Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...
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Actin Polymerization01:42

Actin Polymerization

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Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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微流体インターフェロメトリーを用いた界面ポリメリゼーション運動の測定

Arash Nowbahar1, Vincent Mansard2, Jodi M Mecca3

  • 1Department of Chemical Engineering , University of California , Santa Barbara , California 93106 , United States.

Journal of the American Chemical Society
|February 13, 2018
PubMed
まとめ

ポリアミド薄膜の界面ポリメリゼーション運動を測定した. 新しい微流体干渉測定法により,初期反応速度を制御する反応-拡散境界層が明らかになった.

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Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
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関連する実験動画

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科学分野:

  • ポリマー化学
  • 材料科学
  • 化学工学

背景:

  • インターフェイスポリメリゼーションは繊維,カプセル,フィルムなどの様々な材料の生産に不可欠です.
  • 界面ポリメリゼーションの正確な運動測定は,薄膜と急速な反応のために困難です.

研究 の 目的:

  • 先進的な技術を用いてポリアミド膜形成の反応運動を調査する.
  • 界面ポリメリゼーションにおける薄膜と急速な反応率に関連する測定課題を克服する.

主な方法:

  • 反応界面でのモノメア濃度プロファイルをモニターするために,マイクロ流体干渉計を使用した.
  • ポリメリゼーション中のダイナミックな変化を捉えるために,in-situ測定技術を適用した.

主要な成果:

  • ポリアミド膜形成中のインターフェース近くのモノマー濃度プロファイルを測定しました.
  • 初期反応段階の速度制限段階として,有機段階の反応-拡散境界層を特定した.
  • この特定のポリアミド界面ポリメリゼーションシステムの最初の速度定数を取得しました.

結論:

  • マイクロ流体干渉計は,急速な界面ポリメリゼーション運動を研究するための有効な技術である.
  • 反応-拡散境界層は,ポリアミド膜形成の初期段階に大きな影響を与える.
  • この研究は,インターフェイスポリメリゼーションのための基本的な運動データを提供し,プロセスの最適化と材料設計に役立ちます.