主要な神経組織と周辺組織を横断する霊長類の昼間トランスクリプトームアトラス
Ludovic S Mure1, Hiep D Le1, Giorgia Benegiamo1
1Regulatory Biology Laboratory, Salk Institute for Biological Studies, 10010, North Torrey Pines Road, La Jolla, CA 92037, USA.
まとめ
この研究は 64のバビオンの組織にわたる 昼間の遺伝子発現をマッピングし ほとんどの遺伝子のリズムパターンを明らかにしました これらの日々のリズムは組織特有の機能を提供し,昼間活動する動物と夜間活動する動物の間で異なります.
科学分野:
- ゲノミクス
- クロノバイオロジー
- 哺乳類生理学
背景:
- 日常的な遺伝子発現は 日常的な組織機能に影響しますが アトラスは限られています 特に夜間活動しない種はそうです
- 類人猿の昼夜リズムを理解することは 比較生物学と人間の健康にとって 極めて重要です
研究 の 目的:
- バビオン (Papio anubis) の64の組織について,包括的な昼間トランスクリプトームアトラスを作成する.
- 細胞の重要な機能に関与する遺伝子の組織特有のリズム表現を調査する.
- 昼間の霊長類と夜間の動物間の遺伝子発現の時間的組織を比較する.
主な方法:
- 24時間間で2時間ごとにサンプルを採取した 64匹のバビオンの RNA 配列解析
- リズム的な遺伝子発現パターンとその相分布を特定するためのバイオ情報分析.
- 昼と夜の遺伝子発現の時間的組織を比較した分析
主要な成果:
- パピオ・アヌビスから22の脳領域を含む64の組織の昼間トランスクリプトーム・アトラスを生成した.
- タンパク質をコードする遺伝子の 81.7%が 日常リズムで発現していることがわかりました
- 中枢組織と周辺組織における,至るところに発現する遺伝子の特定された組織特有のリズム的発現.
- 夜明けと夕暮れにピーク遺伝子の発現が観察され 夜明け前には静止期が観測される.
結論:
- バビオンのトランスクリプトームは 日々のリズムを広め 組織に機能的な専門性の層を追加します
- 昼間の霊長類の昼間の遺伝子発現パターンは夜間の動物と大きく異なる.
- このアトラスは,霊長類の生物学的過程の時間的調節を理解するための貴重なリソースを提供します.
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