成人マウスの海馬における神経生成の生きたイメージング
Gregor-Alexander Pilz1, Sara Bottes1, Marion Betizeau1,2
1Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland.
Science (New York, N.Y.)
|February 14, 2018
まとめ
ネズミのヒポカンプスの神経幹細胞と原始細胞 (NSPCs) は非対称かつ対称的に分裂し,生涯の神経生成をサポートします. これらの細胞は 失われる前に 増殖期と神経生成期を経験します
科学分野:
- 神経科学
- 幹細胞生物学
- 発達生物学
背景:
- ニューラル幹細胞と原始細胞 (NSPCs) は,大人の哺乳類のヒポカンプスの新しいニューロンの生成に不可欠です.
- NSPCの系統ダイナミクスを理解することは,生涯の神経生成を理解するための鍵です.
研究 の 目的:
- ネズミの海馬における個々のNSPCの分裂パターンと系統進行を調査する.
- NSPCの行動におけるアカイテ-スキュートホモログ1 (Ascl1) プロモーターの役割を明らかにする.
- 新しく生成されたニューロンの統合をヒポカンプス回路に記述する.
主な方法:
- ネズミのヒポカンプスにおける遺伝的に標識された個々のNSPCとその子孫の慢性 in vivo 画像化.
- 細胞分裂 (対称性および非対称性) と細胞運命を2ヶ月間追跡する.
主要な成果:
- Ascl1を発現するNSPCは,限られた対称および非対称の分裂を経験し,神経生成の爆発とそれに続く細胞喪失につながります.
- 予期せぬ不対称な分裂が非放射性膠状NSPCで観察された.
- Ascl1を標識した細胞系は,増殖から神経生成への移行の発達的なプログラムを示しています.
結論:
- NSPC系統の進行は,定義されたプログラムに従って,持続的な海馬神経生成に寄与します.
- この研究は,NSPCによるニューロンの生成と統合の詳細な地図を提供します.
- 発見はヒポカンプスの生涯の神経生成を支えるメカニズムに洞察を与えます
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