アップコンバージョンナノ粒子媒介光遺伝学による近赤外線深層脳刺激

Shuo Chen¹, Adam Z Weitemier², Xiao Zeng³

¹Laboratory for Circuit and Behavioral Physiology, RIKEN Brain Science Institute, Wakoshi, Saitama 351-0198, Japan. shuoshu@gmail.com chmlx@nus.edu.sg tjmchugh@brain.riken.jp.

Science (New York, N.Y.)

|February 14, 2018

まとめ

アップコンバーションナノ粒子は近赤外線を用いた脳の深層の光遺伝学を可能にします神経回路の非侵襲的な光学制御が可能になるのです

科学分野:

神経科学
バイオテクノロジー
材料科学

背景:

光遺伝学では強力な神経回路の調査ができますが, 可視光が組織に浸透できないため制限があります.
脳の深部刺激には侵襲的な方法が必要で神経学的疾患の治療は困難です

研究の目的:

近赤外線 (NIR) による脳深層刺激のためのアップコンバージョンナノ粒子 (UCNP) を用いた新しい光遺伝的アプローチを開発する.
特定の脳領域の神経活動を操作する UCNP媒介 optogeneticsの有効性を示すために.

主な方法:

分子に合わせたUCNPは,NIR光を吸収し,可視光を発するように合成されました.
脳の深部にある神経細胞を刺激する脳の深部にある神経細胞を刺激する脳の深部にある細胞を刺激する
特定の神経回路をターゲットにしました腹部テグメンタル領域,中間隔膜,そして海馬.

主要な成果:

UCNP媒介の光遺伝学は,ドーパミンの放出を成功裏に誘発した.
脳の振動は阻害性ニューロンの活性化によって引き起こされた
発作は海馬刺激細胞の抑制によって静止された.
記憶のリコールが標的神経の活性化によって引き起こされた

結論:

アップコンバーションナノ粒子は脳の深部回路のトランスクレニアル光遺伝的操作に有効な方法を提供する.
このUCNP技術は侵襲性の少ない光神経制御を容易にし神経学的疾患の潜在的遠隔治療への道を開きます

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