組織の硬化は,細胞の集団的移動を in vivo で引き起こすことで形態変異を調整する
Elias H Barriga1,2, Kristian Franze3, Guillaume Charras1,2
1Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Nature
|February 15, 2018
まとめ
組織の硬化は 胚の発達中の集団的な細胞移動を誘導する. ガストルレーション運動によって引き起こされるメソデルマの硬化により,神経の細胞がメカノセンセーションを通じて移動し,メカノセンセーションと形態変異を結びつける.
科学分野:
- 発達生物学
- 細胞メカニズム
- 組織工学
背景:
- 集団的な細胞移動は 発達や病気に不可欠で 分子や機械的なシグナルが関与します
- 組織力学が体内の集団的な細胞移動に及ぼす影響は,まだほとんど研究されていない.
- Xenopus laevisのニューラル・クライスト細胞は 癌の侵入に似た 集団的な細胞移動のモデルとして機能します
研究 の 目的:
- Xenopus laevisのニューラル・クライスト細胞の集団的移住における機械的なシグナルの役割を調査する.
- 組織力学が胚の形態変異に影響を与えるメカニズムを解明する.
- ガストルーレーション,組織力学, 神経頂部細胞の移動との関係を確立する.
主な方法:
- Xenopus laevisの胚の機械的および分子的な操作
- 神経頂部細胞の移動と上皮からメゼンキマへの移行の分析
- インテグリン-ビンキュリン-タリンを含むメカニカセンセーション経路の調査.
主要な成果:
- 頭部の中皮の硬化が先行し,頭部神経の細胞集団の移動を誘発する.
- 神経頂部細胞は,メソデームの硬さを検出するために,インテグリン-ビンキュリン-タリン媒介のメカノセンセーションを使用します.
- ガストゥルレーション中の収束拡張はメソデームの硬さを増加させ,それによって神経の移動を調整する.
結論:
- ニューラル・クライストの集団的移動を開始するには,メソダームの硬化が必要で十分である.
- 組織メカニズム,特に基板の硬さは,集団的な細胞移動と表皮からメゼンキマへの移行を vivo で引き起こすことができます.
- 収束拡張は形態変異の機械的調整者として機能し,変異した組織力学を通じてガストルレーションとニューラルクライストの移動を結びつける.
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