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Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

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関連する実験動画

Updated: Jun 1, 2026

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
09:30

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Published on: July 13, 2017

ラムダ・リプレッサーとラムダ・クロがOR1とOR3をどのように区別するか

A Hochschild, J Douhan, M Ptashne

    Cell
    |December 5, 1986
    PubMed
    まとめ
    この要約は機械生成です。

    細菌のラムダ抑制体とCroタンパク質は,DNAオペレータ配列を区別する. タンパク質の特定のアミノ酸とオペレーターの塩基対が,これらの認識偏好を決定する.

    さらに関連する動画

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
    08:56

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

    Published on: July 17, 2018

    関連する実験動画

    Last Updated: Jun 1, 2026

    Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
    09:30

    Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

    Published on: July 13, 2017

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
    08:56

    Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

    Published on: July 17, 2018

    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • タンパク質とDNAの相互作用

    背景:

    • ラムダ抑制体およびラムダクロタンパク質は,ファグ染色体上の共有されたオペレータ部位に結合する.
    • これらのタンパク質は,特定のオペレーター配列 (OR1およびOR3) に対して明確な結合偏好を示す.

    研究 の 目的:

    • ラムダ・レプレッサーとCroタンパク質の結合特異性の差異の分子基礎を調査する.
    • 主要なアミノ酸残基と,オペレータ配列の区別を担当するDNA塩基対を特定する.

    主な方法:

    • サイト・ディレクテッド・ミュータゲネシスは,オペレータ配列内の塩基対を変更するために使用されました.
    • アミノ酸の置換は,レプレッサーとCroタンパク質の認識ヘリックスとアミノ端末の腕に導入されました.
    • これらの変更がタンパク質とDNAの相互作用に与える影響を判断するために,結合親和性を分析した.

    主要な成果:

    • タンパク質の好みは,認識ヘリックスとアミノ端末アーム (抑制器の場合) の残基5と6によって決定されます.
    • 差別するためのキーDNAオペレータの位置は,位置3 (Cro) と位置5と8 (repressor) です.
    • 特定のアミノ酸-タンパク質と塩基対の相互作用が,抑制剤とCro.の独特の認識能力を支えている.

    結論:

    • この研究は,ラムダ抑制剤とCroが配列特異のDNA結合を達成する分子機構を明らかにしています.
    • これらの発見は,関連するタンパク質が異なるDNA認識プロフィールを進化させる方法のより深い理解に貢献します.
    • 特定された残留物と塩基対は,オペレーターの差別を決定する重要な決定因子です.