小型オリゴメール熱ショックタンパク質のパラログが異なる機能を進化させる構造原理
Georg K A Hochberg1, Dale A Shepherd1, Erik G Marklund1
1Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford OX1 3QZ, UK.
Science (New York, N.Y.)
|February 24, 2018
まとめ
ほとんどの遺伝子複製タンパク質は 関連するタンパク質との自己組み立てを避け, 異なる機能を進化させる. このタンパク質の選択性は,組み立ての階層とモノメアの柔軟性によって達成され,望ましくないヘテロオリゴメリゼーションを防止します.
科学分野:
- 分子生物学
- タンパク質の生化学
- 進化生物学
背景:
- オリゴメリックタンパク質は 細胞機能に不可欠な 組み立てに際して 顕著な選択性を表しています
- 遺伝子の複製から生じるタンパク質は,しばしば異なる役割を果たすように進化する.
- 選択的なタンパク質組成のメカニズムを理解することは 細胞のプロセスを解読する鍵です
研究 の 目的:
- ヘテロオリゴメリゼーションを回避するために,遺伝子複製タンパク質がどのように進化するかを調査する.
- オリゴメリックタンパク質,特に小型の熱ショックタンパク質における選択的組み立ての背後にあるメカニズムを解明する.
- 共同組み立ての回避と 異なるタンパク質の機能の獲得を相関させる.
主な方法:
- 遺伝子複製されたオリゴメアタンパク質の比較分析
- 小型の熱ショックタンパク質パラログの組み立て実験.
- タンパク質のインターフェースと構造の柔軟性の分析
主要な成果:
- オリゴメリックタンパク質のほとんどのパラログは,機能的分散と相関するヘテロオリゴメリゼーションを回避するために進化する.
- 暫定的な中間物質の階層が選択的オリゴメリゼーションを統制する.
- 非インタフェース領域におけるコンフォーマーションの柔軟性は,インタフェースの保存を保ち,共同組み立てを防止します.
- ホモメアパラログは,コアセンブリのエントロピー的な利点に対抗するために,非パラログのホモメアよりも少ないサブユニットを持っています.
結論:
- 遺伝子の複製は,選択的なタンパク質組成の進化を促し,機能的特異化を促進する.
- 暫定的な組み立ての中間物質とモノメアの柔軟性は,ヘテロオリゴメリゼーションを防ぐための重要なメカニズムです.
- パラログの構造的・機能的な差異は,その組立特性と密接に結びついている.
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