自由に動いている動物の深部組織の単細胞生物発光成像
Satoshi Iwano1, Mayu Sugiyama1, Hiroshi Hama1
1Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
Science (New York, N.Y.)
|February 24, 2018
まとめ
研究者は新しい生物発光システムである AkaBLI を設計し,より鮮やかな in vivo 画像を作成しました. この画期的な発見により 生物医学の研究を進めるため 動物の奥深くにある単細胞の 視覚化が可能になりました
科学分野:
- バイオテクノロジー
- 分子生物学
- バイオエンジニアリング
背景:
- 生物発光は,ルシフェラーゼ酵素がルシフェリン基板を触媒にしています.
- 既存の生物発光システムは,深層組織イメージングの明るさと配信能力に制限があります.
研究 の 目的:
- 生物発光イメージングシステムを開発し,輝度と配送能力を向上させる.
- 深い生物学的過程の 非侵襲的な視覚化 in vivo を可能にします.
主な方法:
- のルシフェラーゼの誘導進化を用いた.
- 赤にシフトした,非常に配送可能なルシフェリンのアナログが使用されました.
- 設計されたシステムであるAkaBLIは, in vivo 画像処理能力についてテストされました.
主要な成果:
- AkaBLIは従来のシステムよりも100~1000倍も明るく放射しています
- マウスの肺血管の単一の腫瘍発生細胞を視覚化しました.
- ネズミのヒッポカンプスとマルモセットのストライアタムの神経活動がビデオレートで追跡されました.
結論:
- AkaBLIは生物工学の光源における 重要な進歩を表しています
- このシステムは,科学,医療,工業用途に前例のない能力を提供しています.
- この技術は深層組織,単細胞の可視化と 長期的な神経活動の監視を容易にする.
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