バクテリアシグナルネットワークにおける制御されたストキャスティシティは,環境の急速な変化に対する耐性を可能にします
Jeffrey N Carey1, Erin L Mettert2, Manuela Roggiani3
1Graduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Cell
|March 6, 2018
まとめ
微生物は 変化する環境で生き残るために 賭けのヘッジを使います 酸素はE. coliの変異性遺伝子発現を誘発し,酸素が失われても集団が成長し,微生物の健康状態を高めます.
科学分野:
- 微生物学
- システム生物学
- 遺伝学
背景:
- 微生物集団は 賭けのヘジングを使って ダイナミックな環境に適応します
- ベットヘジングは,現在の状況ではなく,将来の状況に適したフェノタイプを採用するサブ集団を意味します.
- この戦略は予測不可能な環境で 微生物の生存と適性を高めます
研究 の 目的:
- エシェリキア・コリ菌の遺伝子発現の変動に酸素がどのように影響するかを調査する.
- 酸素誘発の賭けヘッジの背後にある規制メカニズムを特定する.
- 遺伝子発現を調節するノイズが 微生物の健康にどう影響するかを理解する
主な方法:
- 異なる酸素濃度下での大腸菌におけるトリメチアミン酸化物 (TMAO) 呼吸器系の遺伝子発現パターンの分析
- IscR転写因子の役割を研究するために遺伝子操作を用いる.
- 遺伝子発現の多様性と平均発現レベルを定量化する.
主要な成果:
- 酸素はTMAOの呼吸器系における変動的発現を誘導する.
- この変動は細胞集団を多様化し,賭けのヘッジを可能にします.
- 転写因子 IscR は,この酸素依存表現の変動性の主要な調節因子として特定されています.
- 酸素は,IscRがTMAO反応システムを抑制し,ストキャスティック効果と異質表現を増加させます.
結論:
- 酸素は,IscR媒介の調節によってE. coliのベットヘジングを誘導する環境的シグナルとして作用する.
- この研究では 集団の健康状態を改善するための 分子騒音の調節メカニズムが明らかになりました
- この適応的戦略は,酸素の喪失などの環境変化に伴う微生物の成長の持続を可能にします.
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