炭酸塩に敏感なフィトトランスフェリンは,藻の高親近性鉄の吸収を制御する
Jeffrey B McQuaid1,2, Adam B Kustka3, Miroslav Oborník4,5
1J. Craig Venter Institute, Microbial and Environmental Genomics, La Jolla, California 92037, USA.
Nature
|March 15, 2018
まとめ
稀少な鉄を吸収するためにISIP2Aと呼ばれるタンパク質を利用し,鉄と炭酸イオンの両方に依存するプロセスです. 海洋酸性化により 海洋生物にとって重要な栄養素の吸収メカニズムが 損なわれる可能性があります
科学分野:
- 海洋生物学
- 生物化学
- 海洋学
背景:
- 鉄は広大な海域の 植物プランクトンに限られた栄養素です
- ダイアトムは重要なユカリオット植物プランクトンで 成長には不安定な鉄が必要です
- 藻類によるピコモラ・ラビル鉄の吸収のメカニズムは以前は知られていなかった.
研究 の 目的:
- 藻類による不安定な鉄の獲得のメカニズムを解明する.
- 鉄の吸収におけるISIP2Aタンパク質の機能を特徴づける.
- 炭酸イオンの作用を調査する
主な方法:
- ゲノム対応のトランスクリプトミクスで 鉄に反応する遺伝子を特定します
- 藻の遺伝子消去と補完実験 藻の遺伝子消去と補完実験
- 離子依存性を評価するために海水炭酸システムの操作.
主要な成果:
- ISIP2Aは,炭酸塩調整された鉄結合を持つフィトトランスフェリンとして特定されました.
- ISIP2Aを削除すると,高親和性の鉄の吸収が低下し,ヒトのトランスファーリンによって回復した.
- 鉄の吸収は,二次的な鉄と炭酸イオンの濃度に依存し,共制限を示した.
結論:
- ISIP2Aは,フィトトランスフェリンとして機能し,藻の高親近性鉄吸収に不可欠です.
- 炭酸イオンの存在は鉄の吸収を制限し,不安定な鉄との相互作用を強調する.
- 炭酸イオンの濃度を下げることで,海洋生態系にとって重要な鉄の獲得経路に悪影響を及ぼす可能性があります.
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