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TRPチャネルトリオは,急性有害な熱感を媒介する

Ine Vandewauw^1,2, Katrien De Clercq^1,2,3, Marie Mulier^1,2

¹Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), Department of Cellular and Molecular Medicine, University of Leuven, Leuven, Belgium.

|March 15, 2018

まとめ

TRPM3,TRPV1,TRPA1という3つの一時受容電位 (TRP) イオンチャネルは,急性有害な熱を感知するために不可欠です. 3つのTRPチャネルが欠けているマウスは熱吸収反応を失い灼傷を防ぎます

科学分野:

神経科学
分子生物学
感覚生物学

背景:

痛みの知覚は痛感性ニューロンに依存しますが有害な熱に対する分子センサーはほとんど知られていません
これらのセンサーを特定することは保護的離脱反応を理解するために極めて重要です

研究の目的:

体感系で有害な熱を検知する分子センサーを特定する
熱による痛みにおける一時受容体ポテンシャル (TRP) イオンチャネルの役割を明らかにする.

主な方法:

遺伝子ノックアウト (Trpv1-/-Trpm3-/-Trpa1-/-トリプルノックアウトマウス) と薬理学的アプローチを用いた.
単離された感覚神経細胞と神経繊維における有害な熱に対する細胞と行動反応を評価した.
寒さや機械的な刺激に対する評価された痛感反応

主要な成果:

急性有害な熱感は,TRPM3,TRPV1,TRPA1のトリアードに依存しています.
これらの3つのチャネルを併せて除去すると,感覚神経細胞と神経繊維の熱反応が選択的に廃止されます.
三重ノックアウトマウスは有害な熱に対する急性離脱反応を欠いたが,他の刺激に対する正常な反応を保持した.

結論:

TRPチャネル (TRPM3,TRPV1,TRPA1) の機能的に冗長なトライアードは,熱によって引き起こされる急性痛みを引き起こします.
この分子のメカニズムは傷害を防ぐために欠陥を許容するシステムです

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