微生物の化学生成のための設計された細胞代謝の光遺伝的調節

Evan M Zhao¹, Yanfei Zhang¹, Justin Mehl¹

¹Department of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, 25 William Street, Princeton, New Jersey 08544, USA.

Nature

|March 22, 2018

まとめ

オプトジェネティクスは酵母における代謝工学の正確な制御を可能にしますこの研究ではイソブタノールのような有用な化合物の生成を促進する光制御回路が導入されます

科学分野:

合成生物学
メタボリック・エンジニアリング
バイオテクノロジー

背景:

エンジニアリングされた代謝経路の最適化には酵素発現レベルとタイミングの正確な制御が必要です
オプトジェネティックツールは複雑なメディア操作なしにリアルタイムで制御するための理想的なソリューションを提供します.

研究の目的:

エンジニアリングされたSaccharomyces cerevisiaeで価値ある製品の生合成を強化するために,光制御された転写の適用を実証する.
細胞代謝の動的制御のための新しい光遺伝的回路を開発し,成長と生産段階の間でシフトします.

主な方法:

光誘発転写と暗闇誘発生産段階のための新しい光遺伝学回路の導入.
発酵中の酵素発現を調節するために周期的な光パルスを実装する.
Saccharomyces cerevisiaeにおけるミトコンドリアのイソブタノール経路の設計.

主要な成果:

光制御による発酵が達成され,細胞は光のみを用いて成長段階から生産段階へと移行する.
イソブタノール (8.49g/Lまで) と2メチル-1-ブタノール (2.38g/Lまで) のマイクロエアロビック産出が実証された.
酵素発現を微調整し,製品の生産量を増加させるため,パルス光を用いた新しいバイオリアクターの動作モードを展示しました.

結論:

代謝経路の光遺伝的制御は代謝工学の強力な新しい戦略を提供します
この方法により精密な発酵制御が可能になり価値ある化合物の生成が大きく促進されます

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