S-Cis Dieneコンフォーメーション:近赤外線の光スイッチ可能な染料とイメージングアプリケーションのための新しいバトクロミックシフト戦略
Hsiang-Jung Chen, Chee Ying Chew, En-Hao Chang
1Institute of Biomedical Engineering and Nanomedicine , National Health Research Institutes , Miaoli 35053 , Taiwan (ROC).
Journal of the American Chemical Society
|March 29, 2018
まとめ
独特のメロシアニン構造を用いた 無電荷の近赤外線 (NIR) 染料を開発しました この染料は,高感度で細胞と生物の特定のタンパク質のイメージングに切り替え可能な光を提供します.
科学分野:
- 有機化学
- バイオメディカルイメージング
- 光光譜法
背景:
- 近赤外線 (NIR) の光染料の開発は,自己光と光散乱の減少のために深層組織イメージングに不可欠です.
- 従来のNIR染料は,しばしば拡張されたπ結合に依存し,それは集積と限られた光安定性につながる可能性があります.
- 先進的なバイオイメージングアプリケーションでは,NIR発射と光スイッチングを達成するための新しいメカニズムが必要です.
研究 の 目的:
- 近赤外線 (NIR) の光性を持つ新しい無電荷メロシアニン染料を設計し合成する.
- 異常なS-cisダイネコンフォマーを用いたバトクロミックシフトの新しいメカニズムを調査する.
- 標的型生物イメージングのための光スイッチ性を持つタンパク質特有の探査機を開発する.
主な方法:
- NIR発射のためのS-cisダイエンコンフォーマーを備えた新しいメロシアニン染料の合成.
- 極性および粘度に応じて光スイッチングを含む染料の光物理的性質の特徴.
- 染料とタンパク質特異リガンド (SNAPタグとhCAII) を結合して標的型プローブを作成する.
- 生体細胞と生物のインビトロ画像実験で,探査機の性能を評価する.
主要な成果:
- NIR吸収と放出を示す新しい無電荷メロシアニン染料が成功して合成されました.
- NIRシフトは,π結合拡張ではなく,前例のない安定したS-cis dieneコンフォームによって達成されました.
- 染料は,環境の極性および粘度に依存する光交換行動を示した.
- タンパク質特異的な探知器は,標的タンパク質 (SNAP-タグ,hCAII) に結合すると,最大300倍の光増強を示した.
- 生体細胞および体内での標的タンパク質の洗浄なしの局所特有のイメージングは,最小限の背景で達成されました.
結論:
- 非従来のS-cisダイエンの形状は,NIR光染料への新しい経路を提供します.
- 開発された無電荷,光交換可能なNIR染料は,高感度,特異性,背景のないバイオイメージングを可能にします.
- この研究は,多様な生物学的応用のための先進的なNIR光探査機の設計のための新しい戦略を提供します.
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