ランダムヘテロポリマーは,異なった環境でタンパク質の機能を保ちます

Brian Panganiban¹, Baofu Qiao², Tao Jiang¹

¹Department of Materials Science and Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.

Science (New York, N.Y.)

|March 29, 2018

まとめ

科学者は新しい合成ポリマーを作り非原生条件下でタンパク質を安定させ溶解させました毒素を生物処理するための酵素を含むプラスチックなどの先進的なバイオマテリアルの開発を可能にします

科学分野:

バイオマテリアル科学
ポリマー化学
バイオテクノロジー

背景:

活性タンパク質を合成ポリマーに組み込むことで新しいバイオマテリアルの可能性が生まれます
タンパク質は通常,標準的なポリマー加工条件下では機能しません.
本質的に乱れたタンパク質を模倣することがこの課題を克服する鍵です

研究の目的:

非原生環境でタンパク質を溶解し安定させる合成ヘテロポリマーを設計する.
タンパク質ベースのアプリケーションのための合成材料と生物学的システムをインタフェースする新しい戦略を作成します.

主な方法:

タンパク質配列の傾向と表面化学パターンの分析
構成と統計的なモノマー分布を最適化した4モノマーランダムヘテロポリマーの設計.
膜タンパク質と酵素を含むプラスチックの細胞フリー合成におけるヘテロポリマーの応用.

主要な成果:

固有の無秩序なタンパク質を模倣するヘテロポリマーを開発し,非原生条件でタンパク質の安定化を可能にします.
細胞フリーで合成された膜タンパク質で輸送するための適切なタンパク質の折り畳みを達成した.
酵素を含んだプラスチックを使って毒素の生物修復が成功していることが証明された.

結論:

ヘテロポリマーにおける統計的モノマー分布を制御することは,タンパク質の安定化のための実行可能な戦略である.
このアプローチにより,様々な用途のための機能的なタンパク質ベースのバイオマテリアルが作られます.
この発見は生物学的機能と合成材料を結びつける新しい道を開きます

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