Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

702
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
702
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

420
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
420
Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

3.5K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para...
3.5K
Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism01:26

Preparation of 1° Amines: Hofmann and Curtius Rearrangement Mechanism

3.9K
The Hofmann and Curtius rearrangement reactions can be applied to synthesize primary amines from carboxylic acid derivatives such as amides and acyl azides. In the Hofmann rearrangement, a primary amide undergoes deprotonation in the presence of a base, followed by halogenation to generate an N-haloamide. A second proton abstraction produces a stabilized anionic species, which rearranges to an isocyanate intermediate via an alkyl group migration from the carbonyl carbon to the neighboring...
3.9K
Preparation of 1° Amines: Azide Synthesis01:22

Preparation of 1° Amines: Azide Synthesis

4.5K
Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
4.5K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

6.3K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.3K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Carrier Frequencies of Medically Actionable Pathogenic Variants in the Russian Population.

International journal of molecular sciences·2026
Same author

A census of anti-CRISPR proteins reveals AcrIE9 and AcrIE13 as inhibitors of the <i>Escherichia coli</i> K12 type IE CRISPR-Cas system.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A natural depsipeptide antibiotic binds the E-site of the bacterial ribosome.

Nature·2026
Same author

RiPP recognition elements evolved to prevent pathway interference through leader peptide discrimination.

Nature communications·2026
Same author

Analysis of a Novel T1-like Phage KanT1 Reveals a Standalone SH3 Domain as a Widespread Component of <i>Drexlerviridae</i> Cell Lysis Module.

International journal of molecular sciences·2026
Same author

Shared structural mechanisms of alternating access between the secondary peptide transporter SbmA and ABC transporters.

Nature communications·2026

関連する実験動画

Updated: Dec 11, 2025

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.0K

変換阻害剤クレブザゾリシンのバイオシンセシス 単一のYcaO酵素によって触媒化されたヘテロサイクリングとN端のアミジン形成

Dmitrii Y Travin1,2, Mikhail Metelev2,3, Marina Serebryakova2,4

  • 1Department of Bioengineering and Bioinformatics , Lomonosov Moscow State University , Moscow , 119992 , Russia.

Journal of the American Chemical Society
|March 31, 2018
PubMed
まとめ

クレブサゾリシン (KLB) は新しいペプチド抗生物質です. その成熟酵素であるKlpDは,独特にヘテロサイクルとN端のアミジン環の両方を形成し,ペプチドの改変の可能性を拡大します.

さらに関連する動画

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
07:12

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Published on: July 17, 2020

6.5K
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
09:50

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

9.7K

関連する実験動画

Last Updated: Dec 11, 2025

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.0K
Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
07:12

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Published on: July 17, 2020

6.5K
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
09:50

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

9.7K

科学分野:

  • 微生物学
  • 生物化学
  • 分子生物学

背景:

  • クレブサゾリシン (KLB) は,クレブシエラ肺炎のペプチド抗生物質である.
  • KLBはバクテリアのリボソームの 脱出トンネルをターゲットにします
  • その構造は,N端のアミジン環と4つのアゾールヘテロサイクルを特徴としています.

研究 の 目的:

  • クレブサゾリシン (KLB) の成熟メカニズムを解明する.
  • KlpD酵素のバイ機能的役割の特徴を明らかにする.
  • KLBの独特の構造モチーフの形成を理解する.

主な方法:

  • KLBの生産のための in vitroシステム.
  • 生物化学分析でKlpDの活性が確認される
  • 分裂酵素を特定するためのプロテアゼ測定法

主要な成果:

  • KlpDはYcaOドメインの酵素であり,二機能であり,ヘテロサイクル形成とN端のアミジン環合成を触媒する.
  • アミジン環の形成は,TldD/Eによるプロ-KLBのタンパク質分解と同時に行われます.
  • KlpDは,YcaO酵素ファミリーの中で前例のない二重改変能力を示しています.

結論:

  • KlpDの2つの機能は,KLBの成熟に不可欠です.
  • この発見はペプチド抗生物質の 生合成のための新しいメカニズムを明らかにしています
  • KlpDの汎用性は,新しいペプチド改変の設計の可能性を提供します.