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経路特異酵素発現ステキオメトリーの進化的収束

Jean-Benoît Lalanne1, James C Taggart2, Monica S Guo2

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Cell
|April 3, 2018
PubMed
まとめ

生物学的経路は 遺伝子調節が異なる場合でも 精密なタンパク質の量が必要です この研究は,様々な細菌と酵母菌のタンパク質ステキオメトリーを保存し,経路構築の基本的な原理を強調しています.

キーワード:
酵素発現ステキオメトリーレンドセク末端に濃縮されたRNA-seqオペロン進化リボソームプロファイリング

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科学分野:

  • 分子生物学
  • 進化生物学
  • システム生物学

背景:

  • 遺伝子の調節は従来,共発現したタンパク質が同様に調節されていると仮定している.
  • 共同調節経路のための精密なタンパク質ステキオメトリーの必要性は,ほとんど未調査のままである.
  • タンパク質の多量調整を理解することは 生物学的経路の組み立てを理解するために重要です

研究 の 目的:

  • 広大な進化の時間尺度における 細菌の経路における タンパク質のステキオメトリーと 制御戦略を調査する.
  • 異なる遺伝子構造にもかかわらず,保存されたタンパク質比が維持されるかどうかを判断する.
  • ユカリオットにおける経路発現の収束進化を調査する.

主な方法:

  • 異なった細菌のタンパク質ステキオメトリーとオペロン構造の大規模分析 (0.6~20億年).
  • シングル・ヌクレオチド解像度トランスクリプトの多量化のために,エンド・エンリッチドRNA-シーケンシング (Rend-seq) を利用した.
  • 酵母における細菌経路と機能的に類似する経路の比較分析

主要な成果:

  • 保存されたバクテリアの経路で観察されたトランスクリプトの豊富さと遺伝子クラスターの構造の有意な差異.
  • トランスクリプション後のメカニズムは,好ましいタンパク質合成率を維持し,規制の相違を補償する.
  • イーストの独立した進化経路は 同じ経路内の発現パターンに収束しました

結論:

  • 生物学的経路には,規制戦略に関係なく,正確なタンパク質ステキオメトリーが必要である.
  • この発見は 自然システムと合成生物学の両方に 適用可能な 生物学的経路の構築の基本原理を示しています
  • 進化は経路の機能に 精密なタンパク質比率を保証するメカニズムを好みます