モデルプロトセル内の混合配列RNAテンプレートの複製

Derek K O'Flaherty¹, Neha P Kamat^1,2, Fatima N Mirza¹

¹Howard Hughes Medical Institute, Department of Molecular Biology, and Center for Computational and Integrative Biology , Massachusetts General Hospital , Boston , Massachusetts 02114 , United States.

Journal of the American Chemical Society

|April 3, 2018

まとめ

研究者は,シトラートケラテッドマグネシウムを使用して,RNAオリゴーマーに対する脂肪酸ベジケルの浸透性を強化した. この進歩は,原細胞内の非酵素的RNA複製を容易にし,細胞生命の起源を理解するための重要なステップです.

科学分野:

生命の研究の起源
プリバイオティック化学
プロトセル研究

背景:

脂肪酸ベジクル内の化学的なRNA複製は,アビオゲネシスの妥当なステップである.
プロトセルはRNA複製のために核酸と短いオリゴマーを輸入した可能性がある.
以前の研究では,ヘルパーRNAオリゴーマーが溶液中の非酵素RNA複製を可能にすることが示された.

研究の目的:

脂肪酸ベシクルへのRNAオリゴーマー浸透性を高める方法の研究.
特定の条件が原細胞内の非酵素RNA複製をサポートするかどうかを判断する.
ダーウィンの進化に適した原細胞の開発を進める

主な方法:

非酵素のプライマーの拡張のための触媒として,シトラートケラートマグネシウム (Mg2+) を利用した.
異なる長さのRNAオリゴーマーに対するMg2+の脂肪酸膜浸透性を評価した.
短いオリゴヌクレオチドの選択的膜浸透性に対する温度の影響を調査した.

主要な成果:

酸塩基化Mg2+は,膀の破壊なしにRNAオリゴーマーまで膜の透過性を高めました.
短いオリゴヌクレオチドの選択的浸透性は,温度上昇によりさらに改善された.
膀内の4つのヌクレオチドを含むテンプレートの非酵素RNA複製を達成した.

結論:

マグネシウムで強化された膜透過性は,原細胞内のRNA前駆体への封じ込めに不可欠です.
制御された浸透性は,初期の細胞構造の中で遺伝物質の非酵素的複製を可能にします.
この研究は進化の過程を実行できる原細胞を構築する上で重要な一歩を踏み出しています

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