ヘルペス・シンプレックスウイルス1カプシドと関連テグメントタンパク質複合体の構造

¹Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Science (New York, N.Y.)

|April 7, 2018

まとめ

ヘルペス・シンプレックスウイルス (HSV) は,そのカプシド関連テグメント複合体 (CATC) を輸送に使用します. この研究は,HSV-1 CATCの構造を明らかにし,ウイルス感染とゲノム伝達に不可欠なタンパク質の相互作用を詳細に説明しています.

科学分野:

構造生物学
ウイルス学
分子生物学

背景:

ヘルペス・シンプレックスウイルス (HSV) は,ウイルスカプシドの効率的なアクソナル輸送のためにカプシド関連テグメント複合体 (CATC) を必要とします.
CATCの構造を理解することは,HSVの感染メカニズムを明らかにするために不可欠です.

研究の目的:

高解像度冷凍電子顕微鏡でHSV-1カプシドの構造をCATCで決定する.
輸送に関わるキーカプシドとテグメントタンパク質の原子モデルを生成する.

主な方法:

クリオ電子顕微鏡 (cryo-EM) で3.5アングストームの解像度.
HSV-1カプシドタンパク質 (VP5,VP19c,VP23,VP26) とテグメントタンパク質 (pUL17,pUL25,pUL36) の原子モデル構築

主要な成果:

HSV-1 カプシドとCATCの詳細な構造が解明されました.
原子モデルは,特定のタンパク質相互作用 (pUL17,pUL25,pUL36) によるカプシド頂点におけるCATCの異型ペンタミクスの組成を明らかにした.
保存されたタンパク質の折りたたみと,HSV-1カプシドタンパク質の有意な形状の多様性を特定した.

結論:

CATCの構造は,核脱出と軸索輸送におけるその役割についての洞察を提供します.
HSV-1カプシドタンパク質は,驚くべき構造的可塑性を示し,テグメントの相互作用とゲノム包装を容易にする.

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