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Thermincola ferriaceticaのアノドバイオフィルムのpH依存性は,陽子依存の電気化学反応を示唆する
Bradley G Lusk1,2, Isaias Peraza1, Gaurav Albal1
1Swette Center for Environmental Biotechnology , The Biodesign Institute at Arizona State University , P.O. Box 875701, Tempe , Arizona 85287 , United States.
Journal of the American Chemical Society
|April 14, 2018
まとめ
この研究では,pHがアノド呼吸細菌 (ARB) の主要なエネルギー制限因子であることを明らかにした. Thermincola ferriaceticaのバイオフィルムは,陽子依存の電子移転を示し,pHグラデーションが熱力学的にARB呼吸を制限することを示唆しています.
科学分野:
- 微生物学
- 電気化学
- バイオエネルギー
背景:
- アノド呼吸細菌 (ARB) は,微生物の代謝と細胞外電子移転 (EET) の理解に不可欠です.
- ARBの電気化学反応のモニタリングは,呼吸過程の速度制限を明らかにします.
- ARBの機能の鍵となるのは 電子機器と生化学的プロセスを 接続することです
研究 の 目的:
- Thermincola ferriaceticaのアノドバイオフィルムにおける陽子依存の電子移転反応を調査する.
- pH,電子ドナー (アセテート),およびバイオフィルムの成長が電気化学的ポテンシャルに及ぼす影響を決定する.
- ARB呼吸に pH グラデーションによって課される熱力学的制限を理解する.
主な方法:
- 60°Cで研究された熱愛性グラム陽性ARB,Thermincola ferriacetica.
- 異なったpH,アセテットの存在,バイオフィルムの成長条件.
- ターンオーバーでない条件下で測定された電気化学的ミドルポイントポテンシャル (EKA) と公式のリドックスポテンシャル (E°).
主要な成果:
- pHは,T. ferriaceticaのアノドバイオフィルムのエネルギーに影響を与える主要な要因として特定されました.
- E°'の48 ± 7 mV/pHユニットシフトが観察され,陽子依存の速度制限プロセスを示した.
- 結果は,ARBは特に利用可能なエネルギー (<200 mV) を考慮して,pHグラデーションによって熱力学的に制限されていることを示唆しています.
結論:
- 陽子輸送の制限とpHのグラデーションはARBのエネルギーに大きく影響する.
- 観察されたネルンシュタインの行動は,pHによって影響され,陽子転移膜タンパク質とのリンクを示唆している.
- EKAとE°'はpHに依存し,特定の総pHでこれらの値を報告することが推奨される.
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