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関連する概念動画

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.3K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.0K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
pre-mRNA Processing02:01

pre-mRNA Processing

57.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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関連する実験動画

Updated: Feb 12, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
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mRNA構造は,ポリQ駆動の相分離の特異性を決定する.

Erin M Langdon1, Yupeng Qiu2, Amirhossein Ghanbari Niaki2

  • 1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Science (New York, N.Y.)
|April 14, 2018
PubMed
まとめ

メッセンジャーRNA (mRNA) は,その構造を用いて自己結合し,異なる細胞区画を形成する. タンパク質の相互作用によって これらのRNA駆動の液体区画が維持され 細胞の多様性が生まれます

さらに関連する動画

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring
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Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring

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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

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関連する実験動画

Last Updated: Feb 12, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

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Published on: September 29, 2021

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Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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科学分野:

  • 細胞生物学
  • 分子生物学
  • 生物化学

背景:

  • 細胞膜のないコンパートメントは,液体-液体相分離 (LLPS) によって形成されます.
  • これらのRNAに富んだコンパートメント内の異なる分子組成を確立するメカニズムは不明である.

研究 の 目的:

  • 伝達 RNA (mRNA) が異なる液体区画の形成と維持にどのように貢献するか調査する.
  • これらのコンパートメントからの分子募集と排除におけるRNA二次構造の役割を決定する.

主な方法:

  • RNA二次構造によって誘発されるmRNA自己結合を研究した.
  • RNA構造とダイナミクスに対するポリQタンパク質Whi3の影響を調査した.
  • RNA配列に依存する分子変動を分析した.

主要な成果:

  • RNA二次構造はmRNAの自己結合を可能にし,コンパートメントの構成を決定する.
  • タンパク質Whi3はRNAの構造変化を誘導し,分子変動を調節する.
  • 構造に基づくRNA-RNA相互作用は,異なるドロップレットアセンブリとアイデンティティの維持を促します.

結論:

  • RNAの形状と構造は,多様で共存する RNAに富んだ液体コンパートメントの形成に不可欠です.
  • タンパク質媒介によるRNA安定化コンパートメントのダイナミクス
  • これは細胞膜のないコンパートメントの異質性を理解するための枠組みを提供します.