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関連する概念動画

Subcellular Fractionation01:32

Subcellular Fractionation

8.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.9K
Naturalistic Observations02:30

Naturalistic Observations

17.5K
If you want to understand how behavior occurs, one of the best ways to gain information is to simply observe the behavior in its natural context. However, people might change their behavior in unexpected ways if they know they are being observed. How do researchers obtain accurate information when people tend to hide their natural behavior? As an example, imagine that your professor asks everyone in your class to raise their hand if they always wash their hands after using the restroom. Chances...
17.5K
Observational Learning01:12

Observational Learning

1.0K
Albert Bandura's observational learning, also known as imitation or modeling, occurs when a person observes and imitates another's behavior. It is a quicker process than operant conditioning. A well-known example is the Bobo doll study, where children who saw an adult acting aggressively towards the doll were more likely to act aggressively when left alone, compared to those who observed a nonaggressive adult. Many psychologists view observational learning as a form of latent learning...
1.0K
Observational Studies01:11

Observational Studies

11.1K
Observational studies are a type of analytical study where researchers observe events without any interventions. In other words, the researcher does not influence the response variable or the experiment's outcome.
There are three types of observational studies – Prospective, retrospective, and cross-sectional.
Prospective Study
Prospective studies, also known as longitudinal or cohort studies, are carried out by collecting future data from groups sharing similar characteristics. One...
11.1K
Actor-Observer Effect01:23

Actor-Observer Effect

409
The actor-observer effect, a cognitive bias closely linked to the fundamental attribution error, refers to the tendency for individuals to attribute their behavior to external, situational factors while explaining others’ behavior in terms of internal, dispositional traits. This asymmetry in attribution significantly influences social perception and judgment.Cognitive Mechanisms Behind the EffectTwo primary psychological mechanisms contribute to the actor-observer effect: differences in...
409
Levels of Organization01:09

Levels of Organization

141.7K
Biological organization is the classification of biological structures, ranging from atoms at the bottom of the hierarchy to the Earth's biosphere. Each level of the hierarchy represents an increase in complexity that builds upon the previous level.
Molecules Are Composed of Atoms, and Biomolecules Are Assembled from Molecules:
The most basic levels include atoms, molecules, and biomolecules. Atoms, the smallest unit of ordinary matter, are composed of a nucleus and electrons. Molecules...
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Updated: Feb 11, 2026

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
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細胞を本来の状態で観察する:多細胞生物における亜細胞動態のイメージング

Tsung-Li Liu1, Srigokul Upadhyayula1,2,3,4, Daniel E Milkie1

  • 1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

Science (New York, N.Y.)
|April 21, 2018
PubMed
まとめ

この研究は,非侵襲的,高解像度の細胞下ダイナミクスのインビボイメージングのための新しい顕微鏡技術を導入します. この技術は,生理学的環境における細胞の現象的多様性と適応を明らかにする.

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Imaging Subcellular Structures in the Living Zebrafish Embryo
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Imaging Subcellular Structures in the Living Zebrafish Embryo

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Super-Resolution Live Cell Imaging of Subcellular Structures
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Super-Resolution Live Cell Imaging of Subcellular Structures

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08:24

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

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Imaging Subcellular Structures in the Living Zebrafish Embryo
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科学分野:

  • 細胞生物学
  • 顕微鏡検査
  • バイオ物理学

背景:

  • サブセルラーダイナミクスを研究するには,ネイティブの細胞環境内での in vivo イメージングが必要です.
  • 高度な時空解像度と最小限のストレスは,細胞過程の正確な観察に不可欠です.

研究 の 目的:

  • 細胞内動態を観察するための非侵襲的なイメージング技術を開発する.
  • 細胞と周囲の高解像度画像を 偏差なく撮影する.

主な方法:

  • 格子光シート顕微鏡と適応光学の組み合わせ
  • 大量の多細胞体に対して in vivo 画像を用いる.

主要な成果:

  • サブセルラープロセスの非侵襲的,異常のないイメージングが成功しました.
  • エンドサイトーシス,ミトーシス中のオーガネルの改造,細胞の移動 (軸索,免疫細胞,癌細胞) の観察.
  • 生物と発達段階における表型多様性を明らかにした.

結論:

  • 開発された技術は 細胞の行動に関する前例のない洞察を可能にします
  • 生理学的な環境への適応における細胞内在の多様性の役割を強調する.
  • 病気の進行と細胞の適応を理解するための潜在的な応用