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関連する概念動画

Immunofluorescence Microscopy01:12

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Two-Dimensional Microscopy in Microbiology01:29

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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Overview of Microscopy Techniques01:22

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Three-Dimensional Microscopy in Microbiology01:28

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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もっと 見る: 拡張 顕微鏡 の 将来

Devin P Sullivan1, Emma Lundberg1

  • 1Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH - Royal Institute of Technology, Stockholm, 171 21, Sweden.

Cell
|April 21, 2018
PubMed
まとめ

ラベルなしの顕微鏡画像は 細胞の種類や状態などの特徴を予測できます ディープラーニングは 低コストのラベルフリーイメージングから 計算式マルチプレキシングを可能にし 細胞分析を進めています

科学分野:

  • 細胞生物学
  • コンピュータ画像
  • 機械学習

背景:

  • 顕微鏡は細胞生物学の研究に不可欠です.
  • 伝統的な方法では 光ラベルが必要で 費用がかかり 細胞の機能に 障害が生じます
  • 詳細な細胞分析のために,費用対効果が高く,侵襲性の少ない方法が必要である.

研究 の 目的:

  • 細胞情報を予測するためのラベルフリー顕微鏡画像の可能性を調査する.
  • ラベルのない画像から豊富な生物学的データを抽出できる ディープラーニングの枠組みを開発する.
  • ラベルフリー顕微鏡を用いた計算式複合試験の実現可能性を実証する.

主な方法:

  • 細胞のラベルフリー顕微鏡画像に適用された ディープラーニングのフレームワークを利用した.
  • 細胞タイプ,細胞状態,および臓器細胞分布に関連する光ラベルを予測するためにモデルを訓練した.
  • モデルの予測精度を検証した.

主要な成果:

  • ラベルのない細胞画像は,光ラベルを予測するのに成功しました.
  • ディープラーニングモデルは 細胞の種類,状態,臓器の分布を正確に推測しました
  • 単一のラベルフリー画像から複数の生物学的特徴を予測する能力を示した.

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結論:

  • レーベルフリー顕微鏡は ディープラーニングと組み合わせて 伝統的な光レーベルに 強力な代替手段を提供します
  • このアプローチにより,計算式複合検査が可能になり,コストと複雑性が大幅に削減されます.
  • この発見は 生物学的研究において 高濃度で安価な細胞分析のための 新たな道を開きます