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NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

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A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
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Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
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Band Theory

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When two or more atoms come together to form a molecule, their atomic orbitals combine and molecular orbitals of distinct energies result. In a solid, there are a large number of atoms, and therefore a large number of atomic orbitals that may be combined into molecular orbitals. These groups of molecular orbitals are so closely placed together to form continuous regions of energies, known as the bands.
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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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Strong Acid and Base Solutions03:22

Strong Acid and Base Solutions

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A strong acid is a compound that dissociates completely in an aqueous solution and produces a concentration of hydronium ions equal to the initial concentration of acid. For example, 0.20 M hydrobromic acid will dissociate completely in water and produces 0.20 M of hydronium ions and 0.20 M of bromide ions.
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In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
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Updated: Feb 11, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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ハンティングチンエクソン-1ペプチドと脂質ベースのミセラナノ粒子の相互作用は,溶液NMRとQバンドパルスEPRで探査された.

Alberto Ceccon1, Thomas Schmidt1, Vitali Tugarinov1

  • 1Laboratory of Chemical Physics , National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda , Maryland 20892-520 , United States.

Journal of the American Chemical Society
|May 5, 2018
PubMed
まとめ

脂質ミセルはハンティングチンペプチドと相互作用し,螺旋状の形成と結合を促進する. メチオニンの酸化による この相互作用は ハンチントン病のメカニズムに 洞察を与えてくれます

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NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
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Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
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Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

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科学分野:

  • 生物化学
  • バイオ物理学
  • 神経科学

背景:

  • ハンチントン病に ハンチントンエクソン1ペプチドが関与しています
  • 脂質ベースのナノ粒子はタンパク質の集積に影響を与える.

研究 の 目的:

  • ハンティングチンエクソン-1ペプチド (httNTQ7とhttNTQ10) と脂質ミセルの相互作用を特徴づける.
  • これらのペプチドの構造的変化と結合傾向を明らかにする.

主な方法:

  • 核磁気共鳴 (NMR) 化学交換飽和移転 (CEST)
  • 円形の二重化 (CD)
  • パルスQ帯電子パラマグネット共振 (EPR)

主要な成果:

  • ミセルのペプチド結合は,KD約0. 5−1 mMのミリ秒時間スケールで発生する.
  • ミセル結合ペプチドは,残基1〜15で螺旋状の形状を採用する.
  • メチオニンの酸化は結合親和性を低下させ,螺旋の長さを増加させる.
  • モノメール-ジメルの均衡はミセル表面に存在し,ジメルの平行ヘリックス方向性がある.

結論:

  • 脂質ミセルはハンティングチンペプチドの螺旋構造を促進する.
  • ミセル結合とそれに続く二酸化物形成は,タンパク質の結合を促進する.
  • これらの発見は,病気の病原性に関連したハンティングチンの集積の初期段階についての洞察を提供します.