非正規のUbiquitinationに対する構造的洞察は,SIDEによって触発される

Yong Wang¹, Miao Shi¹, Han Feng¹

¹CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Cell

|May 8, 2018

まとめ

レジオネラ・プネモフィラ

科学分野:

分子生物学
構造生物学
生物化学

背景:

ユビキチネーションはユーカリオットの重要なシグナル伝達プロセスです
従来のユビキチネーションはATP依存のE1-E2-E3酵素カスケードを使用する.
レジオネラ・プネモフィラ・サイドエフェクターは,新しいユビキチネーションメカニズムを使用します.

研究の目的:

Legionella SidEによる非正規のユビキチネーションの分子メカニズムを解明する.
SidEのユニークなユビキティナーション活動の構造的基盤を決定する.

主な方法:

X線結晶学で,Side の構造を単独で,Ubiquitin,NAD,ADP-リボースと複合的に決定する.
SidEの酵素活性と基質の認識を分析する生化学分析

主要な成果:

結晶構造は,リガンド結合の前と後の異なるSIDE形状を捕捉した.
構造は,SideのmARTとPDEドメインによるユビキチン転送のユニークな特徴を明らかにした.
SidEファミリーのメンバーは,特定の基板タンパク質の折りたたみ認識が欠けている.

結論:

SidEはホスト機械とは独立した非正規のユビキチネーション経路を使用しています.
構造的および生化学的なデータは,この新しいユビキチネーションシステムに対する機械的洞察を提供します.
この研究は,ユビキチネーションの多様性とバクテリアのエフェクターメカニズムに関する理解を広げています.

キーワード:

ADPリボシライゼーション ADPリボシルトランスフェラーゼレジオネラ・プネモフィラ SDEA について SidE について宿主と病原体の相互作用フォスフォディエステラーゼ構造ユビキチンユビキチネーション

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