関連する実験動画
Updated: Aug 7, 2026

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An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
循環型AMP反応性DNA結合タンパク質:クローンされた胎盤のcDNAに基づく構造
J P Hoeffler1, T E Meyer, Y Yun
1Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Boston.
まとめ
研究者らは,遺伝子転写を調節するDNA配列に結合するサイクルAMP反応性要素結合タンパク質 (CREB) を特定した. このCREBタンパク質は,細胞プロセスに関わる他の転写因子と構造的に類似しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- 循環型AMP (cAMP) は,細胞内第2伝達体として重要な役割を果たします.
- cAMPは,多数の細胞遺伝子の転写を調節する.
- 特定のDNA配列であるTGACGTCA (cAMP反応性要素,CRE) は,この転写調節を媒介する.
研究 の 目的:
- CRE配列に結合するタンパク質を分離し,特徴づけること.
- cAMP媒介による遺伝子調節に関与する特定のCRE結合タンパク質 (CREB) を特定する.
主な方法:
- 放射性CREプローブを使用して,胎盤のラムダ gt11表現ライブラリのスクリーニング.
- CRE結合タンパク質をコードするcDNAの分離と特徴付け.
主要な成果:
- CRE結合特性を持つ326アミノ酸タンパク質をコードするcDNAが分離されました.
- 特定されたタンパク質,CREBは,ルシンのジッパー状の配列に隣接する基本的な領域を有し,DNA結合とタンパク質とタンパク質の相互作用に関与しています.
- CREBには酸性N末端領域があり,トランスクリプション活性化ドメインとして機能する可能性があります.
- CREBのDNA結合ドメインは,c-junと酵母GCN4転写因子のドメインと構造的同質性を示しています.
結論:
- 新しいCRE結合タンパク質 (CREB) が特定され,特徴づけられました.
- CREBは,cAMP媒介の遺伝子転写に作用する.
- CREBの構造的特徴は,異なる種と経路でDNA結合と転写調節のための保存されたメカニズムを示唆しています.
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