古代 の 呼吸 器 の 構造
Hongjun Yu1, Chang-Hao Wu2, Gerrit J Schut2
1Cryo-EM Structural Biology Laboratory, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Cell
|May 15, 2018
まとめ
研究 者 たち は 膜 に 結合 し た 水素 酵素 (MBH) の 構造 を 決定 し,その 進化 的 な 関連 を 複合体 I と 明らかに し まし た. この酵素は,極端な熱下でのATP合成のためにユニークなナトリウムイオンチャネルを使用します.
科学分野:
- 生物化学
- 構造生物学
- 微生物学
背景:
- 膜結合ヒドロゲンゼ (MBH) と複合体Iは同類の呼吸器酵素である.
- 進化の関係に関する 詳細な構造的な理解は 欠けています
研究 の 目的:
- Pyrococcus furiosusの14サブユニットMBHの冷凍電子顕微鏡 (冷凍EM) 構造を解明する.
- MBHと複合体Iの進化的つながりの構造的基礎を提供すること.
主な方法:
- 凍結電子顕微鏡 (cryo-EM) を用いてMBHの構造を決定した.
- MBHと複合体Iを比較した構造分析が行われました.
主要な成果:
- MBHの構造は,複合体IのQモジュールに似た膜固定ヒドロゲネーゼモジュールを示しています.
- イオン転位モジュールは,異なるH+とNa+転位ユニットを備えています.
- 複合体Iに存在しないNa+転位単位は,高温でATP合成のための水素進化とNa+グラデント形成を促進する.
結論:
- MBH構造は,建築的な違いにもかかわらず,複合体Iとの進化的関係を明確にします.
- MBHは,高熱性の条件下でATP合成のためのユニークなNa+グラデーションメカニズムを使用します.
- Mrpアンチポーターの構造とMBHの進化についての洞察が提供されています.
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