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Live Cell Imaging of Chromosome Segregation During Mitosis
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ExTzBox: 生きている細胞のイメージングのための輝くサイクロファン

Indranil Roy, Sharan Bobbala, Jiawang Zhou

  • 1Institute of Molecular Design and Synthesis , Tianjin University , 92 Weijin Road , Nankai District , Tianjin 300072 , P. R. China.

Journal of the American Chemical Society
|May 18, 2018
PubMed
まとめ

生体細胞の画像を撮るための新型光探査機 ExTzBox·4X を開発しました この明るく 細胞毒性のない探査機は 細胞を光白化したり 傷つけることなく 効果的に照らし 探査機設計における以前の課題を克服します

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科学分野:

  • 化学生物学
  • 分子イメージング
  • 材料科学

背景:

  • 生きている細胞のイメージングのための理想的な光探査機を開発するには,明るさ,低細胞毒性,効率的な細胞配送が必要です.
  • 合成フッ素素の設計は,すべての望ましい特性を同時に達成する上で課題に直面しています.
  • 既存の探査機は,細胞毒性または光白化を示す可能性があり,長期の生細胞研究における有用性を制限する.

研究 の 目的:

  • 活細胞画像の新種の光探査機を設計し,合成し,明るさを高め,細胞毒性を低下させる.
  • 新しい箱状のハイブリッドサイクロファンの 光物理的性質と細胞の吸収を調査する
  • コンフォカル顕微鏡を用いた生細胞イメージングアプリケーションにおける開発された探査機の有効性を実証する.

主な方法:

  • 拡張ヴィオゲン (ExBIPY) とディピリジルチアゾロチアゾール (TzBIPY) ユニットを結合することによって,箱状のハイブリッドサイクロファンの合成,ExTzBox·4X (6·4X).
  • 量子収量決定を含む光物理的特徴付け (ΦF = 1.00 for 6·4Cl).
  • RAW264.7マクロファージとコンフォカル顕微鏡を用いた生細胞画像の細胞毒性評価

主要な成果:

  • ExTzBox·4X (6·4Cl) は高量子産出を示し,その硬い構造のために高濃度 (100 μM) で細胞毒性を持たない.
  • コンフォカル顕微鏡では,RAW 264.7マクロファージによる64+の効率的な吸収が確認され,明るい光が生じました.
  • 探査機は,より少ない光性アサイクリック参照化合物とは異なり,光白化や細胞行動の障害を示さなかった.

結論:

  • 硬い箱状のサイクロファンの構造は,生細胞イメージングの高度に光し,細胞毒性のない探査機の開発に重要である.
  • ExTzBox·4Xは生物学的研究のための光探査技術の有望な進歩を表しています.
  • 開発された探査機は,細胞活性を損なうことなく,細胞のプロセスを効果的に長期的に可視化します.