レジオネラエフェクターSdeAによるユビキチン改変の構造的基礎
Yanan Dong1, Yajuan Mu1, Yongchao Xie1
1Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Nature
|May 26, 2018
まとめ
この研究は,レジオネラ SdeAの背後にある構造的メカニズムを明らかにします.
科学分野:
- 分子生物学
- 構造生物学
- 生物化学
背景:
- タンパク質のユビキチネーションは,真核細胞のプロセスを調節する重要な翻訳後の改変である.
- レギオネラエフェクタ SdeAは独特のフォスフォリボシル関連ユビキチネーションを媒介するが,そのメカニズムは不明である.
研究 の 目的:
- SdeA媒介によるユビキチン改変の構造的メカニズムを解明する.
- フォスフォリボシル結合ユビキチネーションの過程を理解する.
主な方法:
- X線結晶学を使用して,SdeAの構造を様々な状態 (リガンドフリー,Ub-bound,Ub-NADH-bound) で決定した.
- ユビキチン結合時のSdeAの構造変化の分析
主要な成果:
- 構造は,SdeAのモノ-ADP-リボシルトランスフェラーゼ (mART) とフォスフォディエステラーゼ (PDE) ドメインが触媒ドメインを形成することを明らかにする.
- ユビキチン結合は,mART領域のARTTとPNループに重要な構造変化を誘導する.
- UbiquitinのArg72は,ADPリボシレーション中に相互作用およびその後のサイドチェーンの動きを開始する探査機として機能する.
結論:
- この研究は,SdeA媒介によるユビキチン改変に関する構造的洞察を提供します.
- この研究は,フォスフォリボシル関連ユビキチネーションメカニズムに関するさらなる研究のための基礎を築く.
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