関連する実験動画
Updated: Aug 1, 2026

08:51
Evaluation of Mammary Gland Development and Function in Mouse Models
Published on: July 21, 2011
まとめ
RAS遺伝子が欠けている酵母胞子は発芽することができません. しかし,哺乳類のRAS遺伝子を導入することで,発芽と成長が可能になり,RASタンパク質の機能が種間で保たれていることが示されています.
科学分野:
- 分子生物学は分子生物学である.
- 進化生物学の進化生物学について
- イースト遺伝学 イースト遺伝学
背景:
- RASタンパク質は,多くの生物の細胞成長と生存能力に不可欠です.
- 固有のRAS遺伝子は,酵母胞子の発芽と植物の成長に不可欠です.
研究 の 目的:
- 酵母におけるRASタンパク質の生化学的機能の本質を調査する.
- 哺乳類RAS遺伝子が酵母RAS遺伝子を代用できるかどうかを判断する.
- RASタンパク質機能の進化的保存を調査する.
主な方法:
- 酵母胞子を利用して,内生的なRAS遺伝子を欠くように遺伝子工学によって設計された.
- ガラクトース誘導性プロモーター (GAL10) の下でキメリックまたは完全な哺乳類のH-RAS遺伝子を導入する.
- ギャラクトースの存在下での胞子発芽とハプロイドの子孫の生存可能性を評価する.
主要な成果:
- 固有のRAS遺伝子が欠けている酵母胞子は発芽できませんでした.
- 哺乳類のRAS遺伝子を導入することで,発芽と生存可能なハプロイドの子孫の生産が救われました.
- 救出された酵母は,ギャラクトーゼに依存した成長と生存能力を発揮した.
結論:
- RASタンパク質の生化学的機能は,植物性ハプロイド酵母にとって不可欠です.
- RASタンパク質のこの重要な機能は,酵母から哺乳類への進化を通して保存されています.
関連する概念動画
The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...

