tRNAの進化過程におけるペアウェイズおよび上位階の遺伝子相互作用
Júlia Domingo1, Guillaume Diss1, Ben Lehner2,3,4
1Systems Biology Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
Nature
|June 1, 2018
まとめ
遺伝的相互作用は高度にダイナミックで,異なる酵母tRNA遺伝子型でポジティブとネガティブな効果を切り替える. このエピスタシスを理解することは,進化する集団における正確な遺伝的予測に不可欠です.
科学分野:
- 遺伝子 と 進化
- 分子生物学
- 酵母遺伝学
背景:
- 遺伝的文脈は突然変異の結果に大きく影響する.
- ペアワイド変異の相互作用は,現象型の変化に寄与する.
- ゲノタイプ間の遺伝的相互作用の安定性はほとんど不明である.
研究 の 目的:
- 遺伝子の相互作用の安定性とダイナミクスを量化する.
- 高いレベルの遺伝子の相互作用を調査する
- 遺伝子背景が変異効果に与える影響を評価する.
主な方法:
- 4万5千以上の遺伝子相互作用の 体系的なマッピングです
- 500種類以上の密接に関連した酵母tRNAの遺伝子型を分析した.
- 配列と高次元のエピスタシスの定量化
主要な成果:
- すべての変異ペアは少なくとも9%の遺伝子背景で相互作用した.
- ゲノタイプ間での相互作用はしばしば陽性から陰性へと変化した.
- 高度な相互作用は豊富でダイナミックでした
- 個々の突然変異は 有害から有益に変化した.
結論:
- 遺伝子の相互作用は高度にダイナミックで 文脈に依存しています
- 遺伝子の正確な予測には 多様な背景の変異効果を測定する必要があります
- 高級エピスタティック用語は予測モデルに不可欠です.
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