DNA結合活動は,AMVおよびE26ウイルスの浄化されたmybタンパク質と関連しており,E26 ts変異体には温度感性である
Cell
|April 1, 1985
まとめ
鳥類骨髄芽細胞症ウイルス (AMV) と鳥類白血病ウイルスE26 (E26) の腫瘍遺伝子タンパク質がDNAを結合する. 変異に影響する変異は,これらのmybタンパク質のDNA結合能力を損なうこともあります.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 腫瘍学 腫瘍学
背景:
- 鳥類骨髄芽細胞症ウイルス (AMV) と鳥類白血病ウイルスE26 (E26) は,それぞれ腫瘍遺伝子のタンパク質,p48v-mybとp135gag-myb-etsをコードする.
- これらのタンパク質は主に骨髄細胞の核に局所されています.
研究 の 目的:
- AMVおよびE26腫瘍遺伝子のタンパク質のDNA結合特性を調査する.
- これらのタンパク質の変換能力とDNA結合能力との関係を決定する.
主な方法:
- タンパク質の局所化を決定するために,間接的な免疫光法が使用されました.
- モノクローナル抗体による免疫親和染色体は,タンパク質の浄化に使用されました.
- タンパク質とDNAの相互作用を評価するために,in vitro DNA結合アッセイが行われました.
主要な成果:
- 浄化されたp48v-mybおよびp135gag-myb-etsタンパク質は,in vitroでDNA結合活性を示した.
- 変異したp135gag-myb-etsタンパク質は,温度に敏感で,DNA結合が減少または熱可動性を示した.
- Myb固有の抗体は,AMVおよびE26腫瘍遺伝子のタンパク質のDNA結合を抑制した.
結論:
- myb腫瘍遺伝子は,細胞の変異と,そのコード化されたタンパク質のDNA結合活動の両方で重要な役割を果たします.
- myb腫瘍遺伝子の内部の病変は,DNAと相互作用する能力に直接影響を与えます.
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