ヒストン変種H2A.Zは,単細胞トランスクリプトミクスによって明らかにされた滑らかな筋肉細胞のアイデンティティを維持するために必要です

Fang Yao¹, Peng Yu¹, Yue Li²

¹State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases (F.Y., P.Y., X.Y., Z.L., F.L., Y.W., D.L., B.Z., L.W.), Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

Circulation

|June 7, 2018

まとめ

ヒストン変種H2A.Zは,血管滑らかな筋肉細胞 (VSMC) のアイデンティティを維持する. H2A.Zの回復はアイデンティティを回復し,ネオインティマの形成を防ぐ.

科学分野:

エピジェネティクス
細胞生物学
心血管研究

背景:

ヒストンの変種はクロマチンの構造と細胞機能を調節する.
血管疾患における細胞のアイデンティティを維持する彼らの役割は,ほとんど未知のままです.

研究の目的:

血管の滑らかな筋肉細胞 (VSMC) のアイデンティティにおけるヒストン変異の役割を調査する.
動脈硬化病変のヒストンの特徴を特定する

主な方法:

病気の人の動脈の単細胞RNA配列解析
ヒストン変異体H2A.Zの占有率と発現率の分析
血管損傷に対するH2A.Z過剰発現のインビボ試験

主要な成果:

H2A.Zは,VSMCマーカー遺伝子の規制領域を占める,VSMCアイデンティティの重要なヒストンシグネチャーです.
H2A.Zの占有量はVSMCの分化時に減少し,核細胞の周回と遺伝子の活性化を促進する.
病気の人の血管組織ではH2A.Zレベルが著しく低下します.
In vivo H2A.Z過剰発現はVSMCのアイデンティティを回復し,傷害後のネオインティマの形成を減少させます.

結論:

ダイナミックなヒストン変異の占有は,細胞運命を決定する新しい規制メカニズムです.
H2A.ZはVSMCのアイデンティティを維持する上で重要な役割を果たしています.
H2A.Zは血管疾患の治療対象となる可能性がある.

キーワード:

ヒストン滑らかな筋肉細胞血管疾患

さらに関連する動画

07:17

Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

Published on: October 30, 2019

7.3K

08:24

Isolation of Murine Coronary Vascular Smooth Muscle Cells

Published on: May 30, 2016

14.8K

関連する概念動画

02:30

Histone Variants at the Centromere

5.1K

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...

5.1K

02:32

Histone Modification

16.2K

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

16.2K

01:23

Functions of Smooth Muscles

3.4K

Smooth muscles are an important type of muscle tissue that plays a vital role in the involuntary movements of internal organs. For example, they help regulate the movement of food through the gut and the flow of blood through the circulatory system.
Function of visceral smooth muscles
Visceral smooth muscle is found in the walls of all hollow organs, except the heart, and is a key player in the involuntary movements that drive the functioning of these internal organs. This tissue is arranged in...

3.4K

01:25

Smooth Muscle Contraction

7.9K

Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...

7.9K

01:14

Maintenance of the ES Cell State

2.8K

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

2.8K

01:13

Structure and Organization of Smooth Muscles

8.8K

Smooth muscle tissue is a type of muscle tissue that can be found lining various vital organs in the human body, including the lungs, blood vessels, digestive tract, and respiratory tract. This type of tissue is responsible for regulating the movements of these organs, playing crucial roles in the functioning of various systems, including the vascular, digestive, respiratory, and urinary systems.
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...